Determination of inorganic cations in fermentation and cell culture media using cation-exchange liquid chromatography and conductivity detection

Robinett, R.S.R.bin; George, H.A.; Herber, W.K.

Journal of Chromatography A 718(2): 319-327

1995


ISSN/ISBN: 0021-9673
DOI: 10.1016/0021-9673(95)00685-0
Accession: 008446193

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Abstract
Reliable methods to quantitate inorganic cations in biological culture media are often helpful for medium optimization and comprehensive process monitoring and control. Analysis of inorganic cations found in culture media was accomplished by ion chromatography for several types of microbial fermentations as well as mammalian and insect cell culture samples. An isocratic method consisting of a Dionex IonPac CS12 analytical column and 4 mM methanesulfonic acid mobile phase was used with suppressed conductivity detection. Sample preparation was a simple dilution of filtered broth with water. The analyses time was 20 min. This analysis accurately monitored eight inorganic cations, several of which are commonly present in fermentation media (Na+, NH-4+, K+, Mg-2+, Ca-2+).