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Differences in cellular transport of tri-iodothyronine and thyroxine: cell cycle-dependent alteration of tri-iodothyronine uptake



Differences in cellular transport of tri-iodothyronine and thyroxine: cell cycle-dependent alteration of tri-iodothyronine uptake



Journal of Endocrinology 147(3): 479-485



Cellular and nuclear uptake of tri-iodothyronine (T-3) and thyroxine (T-4) was examined using the cultured cell line derived from rat liver, clone 9, and rat hepatoma, dRLH-84. The saturable cellular uptake of T-3 and T-4 was demonstrated in these cells. First we examined the cell cycle-dependent alteration of thyroid hormone uptake. Cellular T-3 uptake was minimal in the early G1 phase and increased in the late G1 phase, reaching a maximal level in the S phase. Alterations in nuclear T-3 uptake were in accordance with the changes in cellular T-3 uptake. On the other hand, cellular and nuclear T-3 uptake was unchanged throughout the cell cycle, suggesting the T-3 specificity of the cell cycle-dependent alteration of cellular hormone transport. Next we examined the effect of sodium butyrate on the cellular transport of thyroid hormones. After treatment with 5 mM sodium butyrate, cellular and nuclear uptake of T-3 was increased, reaching a maximal level (four- to sevenfold increase) after 48 h. When cells were incubated for 48 h with various concentrations of sodium butyrate, T-3 uptake was enhanced by 1 mM sodium butyrate, reaching a maximal level with 5 mM. Although cellular T-4 uptake was also increased after treatment with sodium butyrate, the degree and time-course of the increase were different from those of T-3. The maximal increase in cellular T-4 uptake (two- to threefold increase) was attained 20 h after treatment. Despite the increase in cellular T-4 uptake, nuclear T-4 uptake was decreased after treatment with sodium butyrate. For both T-3 and T-4, the enhanced cellular uptake was due to the increased V-max without changes in the Michaelis-Menten constant. These data indicate that cellular transport of T-4 is different from that of T-3 in rat hepatic cells.

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Accession: 008466224

Download citation: RISBibTeXText

PMID: 8543918

DOI: 10.1677/joe.0.1470479



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