EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin


Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human alpha-lactalbumin



Journal of Molecular Biology 253(5): 651-657



ISSN/ISBN: 0022-2836

PMID: 7473740

DOI: 10.1006/jmbi.1995.0579

alpha-Lactalbumin (alpha-LA) is a two-domain, calcium-binding protein that forms one of the best studied molten globules. We present here amide hydrogen exchange studies of the molten globule formed by human alpha-LA at pH 2 and compare these results with a similar study of the native state at pH 6.3. The most persistent structure in the molten globule is localized in the helical domain, consistent with previous results. However, the helices most protected from hydrogen exchange in the molten globule are, in the native state, less protected from exchange than other regions of the protein. The molten globule appears to contain major elements of the native fold, but formation of the fully native state requires stabilization of structure around the calcium-binding site and domain interface.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008468715

Download citation: RISBibTeXText

Related references

Different subdomains are most protected from hydrogen exchange in the molten globule and native states of human a-lactalbumin. Journal of Molecular Biology 253: 1-7, 1995

Comparison of intramolecular packings in human alpha -lactalbumin in native and molten globule states. Studia Biophysica 112(2/3): 207-211, 1986

A comparative study of the alpha-subdomains of bovine and human alpha-lactalbumin reveals key differences that correlate with molten globule stability. Protein Science: a Publication of the Protein Society 14(1): 89-96, 2005

Quasielastic light scattering from human alpha lactalbumin comparison of molecular dimensions in native and molten globule states. International Journal of Biological Macromolecules 8(4): 231-236, 1986

Probing subtle differences in the hydrogen exchange behavior of variants of the human alpha-lactalbumin molten globule using mass spectrometry. Journal of Molecular Biology 311(4): 909-919, 2001

Non two-state denaturing transition from native to molten globule states of apo-alpha-lactalbumin. Biophysical Journal 72(2 Part 2): A394, 1997

Action of protein-glutaminase on alpha-lactalbumin in the native and molten globule states. Journal of Agricultural and Food Chemistry 49(12): 5999-6005, 2001

Direct measurement of the energetics of the molten globule and native states of alpha lactalbumin. Biophysical Journal 61(2 Part 2): A477, 1992

Contribution of the 6-120 disulfide bond of alpha-lactalbumin to the stabilities of its native and molten globule states. Biochemistry 31(50): 12695-12700, 1992

Rotational diffusion studies of native and molten globule states of bovine alpha lactalbumin. Studia Biophysica 126(2): 87-97, 1988

Modulation of stability of the native and molten globule states of alpha-lactalbumin by site-specific mutagenesis. FASEB Journal 8(7): A1386, 1994

Structure and stability of the molten globule state of guinea-pig alpha-lactalbumin: a hydrogen exchange study. Biochemistry 32(21): 5681-5691, 1993

Vibrational Raman optical activity of alpha-lactalbumin: comparison with lysozyme, and evidence for native tertiary folds in molten globule states. Journal of Molecular Biology 254(4): 747-760, 1995

Probing subtle differences in the hydrogen exchange behavior of variants of the human a-lactalbumin molten globule using mass spectrometry. Journal of Molecular Biology 311(4): 9-19, 2001

Organization and dynamics of tryptophans in the molten globule state of bovine alpha-lactalbumin utilizing wavelength-selective fluorescence approach: comparisons with native and denatured states. Biochemical and Biophysical Research Communications 394(4): 1082-1086, 2010