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Differential regulation of endogenous endonuclease activation in isolated murine fibroblast nuclei by ras and bcl-2

, : Differential regulation of endogenous endonuclease activation in isolated murine fibroblast nuclei by ras and bcl-2. Oncogene 10(4): 769-774

Transfection of a murine fibroblast cell line with an activated form of the Harvey ras oncogene conferred sensitivity to apoptosis induced by various agents. This intrinsic sensitivity to apoptosis correlated with the expression of endogenous endonuclease activity in isolated nuclei that was undetectable in the untransfected parental cell line. Subsequent transfection with the human bcl-2 oncogene prevented the morphological and biochemical features of apoptosis in whole cells, although it failed to confer complete protection against cell death. Furthermore, transfection of the bcl-2 oncogene also inhibited the enhanced endonuclease activity in isolated nuclei. Our results indicate that some of the effects of Ha-ras and bcl-2 and potentially other oncogenes, are exerted on the biochemical machinery of apoptosis at the level of the nucleus.

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Accession: 008474467

PMID: 7862455

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Wang, S.L.; Wu Wang, C. Y.; Correa, A.; Brigham, M.; Feng, J.; Slomiany, A.; Slomiany, B. L., 1994: Biochemical characterization of epidermal growth-factor receptor in Syrian hamster buccal pouch. Binding of (125I)-epidermal growth factor (EGF) to the membrane of the adult hamster buccal pouch was specific and protein concentration dependent, and was significantly higher than that of rat buccal tissue (hamster versus rat; 12.1 +- 0.9 versus...

Kinoshita S.; Uzu K.; Nakano K.; Takahashi T., 1971: Mitomycin derivatives part 2 derivatives of decarbamoyl mitosane and decarbamoyl mitosene. Journal of Medicinal Chemistry 14(2): 109-112

Pascoe, F. P., 1887: Notes on Coleoptera, with Descriptions of New Genera and Species. Annals of Natural History, 5(xx): 8-20

Abe F.; Yamauchi T.; Wan A.S.C., 1988: Sesquilignans sesterlignans and trilignans from stems of cerbera manghas and cerbera odollam. Sesqui-, sester- and tri-lignans principally composed of olivil were isolated from the stems of Cerbera manghas and C. odollam and named cerberalignans D-I. The structures were determined from spectral evidence.

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Sherwood J.L.; Hunger R.M.; Keyser G.C.; Myers L.D., 1990: Production of a monoclonal antibody for evaluation of hard red winter wheat cultivars to wheat streak mosaic virus. The monoclonal antibody technology has provided a means to produce a supply of highly specific uniform antibody which is useful in the detection of plant viruses and which facilitates disease resistance screening. Because of the specificity of a m...

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Forbes R. B.; Ragan D. M., 1969: Mafic granulite inclusions in basalts as possible samples of the deep crust. Eos, Transactions, American Geophysical Union 50(4): 344

Popov, A. L.; Gavrilov, G. P., 1976: Methods of training grapevines on a single stem in Moldavia. Several methods of training grapevines on a single stem and their subsequent maintenance up to the 4th and 5th year are discussed and diagrammatically illustrated. Compared with vines trained low stems and covered with soil for winter protection,...