Differential staining of human erythrocyte membrane proteins by coomassie and silver: Implications in the assessment of homogeneity of membrane proteins

Pikula, S.; Bandorowicz-Pikula, J.; Awasthi, S.; Awasthi, Y., C.

Biochemical Archives 12(4): 237-243


ISSN/ISBN: 0749-5331
Accession: 008475472

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Non-uniform staining of electrophoretically separated polypeptides is one of the major problems in the assessment of their homogeneity. In the present communication we report the example of such behavior of human erythrocyte membrane and cytoskeleton proteins, stained with Coomassie brilliant blue R-250, Silver nitrate, and Periodic acid Schiff's (PAS) in reduced denaturing gels.