Discriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis
Johnson, W.M.; Bernard, K.; Marrie, T.J.; Tyler, S.D.
Journal of Clinical Microbiology 32(10): 2620-2621
1994
ISSN/ISBN: 0095-1137 PMID: 7814513 Accession: 008483934
Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.
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