Effect of different positively charged amino acids, carboxyl-terminally of the signal peptidase cleavage site, on the translocation kinetics of a precursor protein in Escherichia coli K-12

Struyve, M.; Bosch, D.; Visser, J.; Tommassen, J.

FEMS (Federation of European Microbiological Societies) Microbiology Letters 109(2-3): 173-178

1993


Accession: 008529289

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
Introduction of positively charged amino acids immediately downstream of the signal sequence in prokaryotic precursor protein is known to affect the export process. However, it is not clear whether different positively charged amino acids affect the export process similarly. To investigate this, the glutamate at position +2 of outer membrane protein PhoE was substituted by arginine, lysine or histidine. Pulse-chase experiments revealed that the Lys and Arg residues at position +2 caused a reduced processing rate, and that the effect was markedly more severe in the case of the Arg residue. Trypsin accessibility experiments revealed that the accumulated precursors were present in the cytoplasm. Since the degree of the inhibitory effect corresponded to the pK-a of the different positively charged amino acid, this suggests that the positively charged residues must be deprotonated during the secretory process.