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Effect of porcine growth hormone and carbadox on growth performances of pigs and in vitro L6 muscle cell proliferation

Effect of porcine growth hormone and carbadox on growth performances of pigs and in vitro L6 muscle cell proliferation

Journal of the Agricultural Association of China 0(168): 125-134

The purpose of this experiment was to investigate the effect of porcine growth hormone (pGH) injection and carbadox treatment on growth performances of pigs and serum insulin-like growth factor-I (IGF-I) and insulin concentration, and in vitro muscle cell proliferation. Twenty-four growing pigs (avg. 30Kg) were randomly assigned to four treatments based on corn and soybean meal, including crude protein content 16%, digestible energy 3400 Kcal/Kg as a control group (treatment 1). Pigs in treatment 2 were given control diet and 75 mu-g/d pGH injection for 9 days starting from 21st day of this experiment. Pigs in treatment 3 were given control diet supplemented with 55 ppm carbadox. Pigs in treatment 4 were given control diet with both 55 ppm carbadox and 75 mu-g/d pGH. Blood samples were taken at 08:30, 09:00, 09:30, 10:00, 10:30, 11:00, 11:30, on days 0, 14, and 30 of this experiment. Serum collected on the same day was pooled by individual pig and frozen at -20 degree C for IGF-I, insulin concentration analysis, and in vitro muscle cell (L6) culture. The results revealed that, after treatment, daily gain (Kg/d) of four treatments was 0.62, 0.66, 0.50, and 0.71 (p lt 0.05), respectively. Feed efficiency (feed/gain) was 2.52, 2.28, 3.17, and 2.19 (P lt 0.05), respectively. Serum IGF-I concentration (ng/ml) was 174, 198, 164, and 254 (p lt 0.05), respectively. The correlation coefficients (r) of IGF-I concentration and daily gain, feed efficiency, and cell number were 0.43 (p=0.008), -0.30 (p=0.07), and 0.42 (p=0.014), respectively. Serum insulin concentration (ng/ml) of the four treatments was 12, 16, 13, and 12 (P lt 0.05) and cell number (x10-3/cm-2) was 31.5, 29.5, 26.7, and 40.2 (P lt 0.05), respectively. In conclusion, growing pigs treated with 75 mu g/d pGH can improve daily gain and feed efficiency, increase serum IGF-I and insulin concentration, and stimulate in vitro muscle cell proliferation. Pigs treated with both carbadox and pGH had additive effect on pig performances, serum IGF-I concentration, and muscle cell number. However, no improvement was seen when pigs were treated with carbadox only in this experiment.

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Accession: 008542842

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