EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Effect of sarcoplasmic reticulum (SR) calcium content on SR calcium release elicited by small voltage-clamp depolarizations in frog cut skeletal muscle fibers equilibrated with 20 mM EGTA


Effect of sarcoplasmic reticulum (SR) calcium content on SR calcium release elicited by small voltage-clamp depolarizations in frog cut skeletal muscle fibers equilibrated with 20 mM EGTA



Journal of General Physiology 112(2): 161-179



ISSN/ISBN: 0022-1295

PMID: 9689025

DOI: 10.1085/jgp.112.2.161

Cut muscle fibers from Rana temporaria (sarcomere length, 3.5-3.9 micro(m); 14-16 degreesC) were mounted in a double Vaseline-gap chamber and equilibrated with an external solution that contained tetraethyl ammonium- gluconate and an internal solution that contained Cs as the principal cation, 20 mM EGTA, and 0 Ca. Fibers were stimulated with a voltage-clamp pulse protocol that consisted of pulses to -70, -65, -60, -45, and -20 mV, each separated by 400-ms periods at -90 mV. The change in total Ca that entered into the myoplasm (Delta[CaT]) and the Ca content of the SR ([CaSR]) were estimated with the EGTA/phenol red method (Pape, P.C., D.-S. Jong, and W.K. Chandler. 1995. J. Gen. Physiol. 106:259-336). Fibers were stimulated with the pulse protocol, usually every 5 min, so that the resting value of [CaSR] decreased from its initial value of 1,700-2, 300 microM to values near or below 100 microM after 18-30 stimulations. Three main findings for the voltage pulses to -70, -65, and -60 mV are: (a) the depletion-corrected rate of Ca release (release permeability) showed little change when [CaSR] decreased from its highest level (>1,700 microM) to approximately 1,000 microM; (b) as [CaSR] decreased below 1,000 microM, the release permeability increased to a maximum level when [CaSR] was near 300 microM that was on average about sevenfold larger than the values observed for [CaSR] > 1,000 microM; and (c) as [CaSR] decreased from approximately 300 microM to <100 microM, the release permeability decreased, reaching half its maximum value when [CaSR] was approximately 110 microM on average. It was concluded that finding b was likely due to a decrease in Ca inactivation, while finding c was likely due to a decrease in Ca-induced Ca release.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008545305

Download citation: RISBibTeXText

Related references

Voltage dependence of sarcoplasmic reticulum calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA. Biophysical Journal 66(2 Part 2): A87, 1994

Effect of partial sarcoplasmic reticulum calcium depletion on SR calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA. Biophysical Journal 72(2 Part 2): A120, 1997

Effect of sarcoplasmic reticulum calcium content on SR CA release elicited by small depolarization in frog cut skeletal muscle fibers. Biophysical Journal 72(2 Part 2): A273, 1997

Effects of partial sarcoplasmic reticulum calcium depletion on calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA. Journal of General Physiology 112(3): 263-295, 1998

Repriming charge movement and sarcoplasmic reticulum calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA. Biophysical Journal 74(2 Part 2): A339, 1998

Calcium release and its voltage dependence in frog cut muscle fibers equilibrated with 20 mM EGTA. Journal of General Physiology 106(2): 259-336, 1995

Dependence of the calcium induced release from the sarcoplasmic reticulum of skinned skeletal muscle fibers from the frog semitendinosus on the rate of change of free calcium concentration at the outer surface of the sarcoplasmic reticulum. Journal of Physiology (Cambridge) 353: 56P, 1984

Measurement of sarcoplasmic reticulum sr calcium release in frog cut muscle fibers with egta and phenol red. Biophysical Journal 61(2 Part 2): A130, 1992

Sarcoplasmic reticulum calcium release in frog cut muscle fibers in the presence of a large concentration of EGTA. Society of General Physiologists Series 51: 255-268, 1996

Reduction of calcium inactivation of sarcoplasmic reticulum calcium release by fura-2 in voltage-clamped cut twitch fibers from frog muscle. Journal of General Physiology 102(2): 333-370, 1993

Calcium buffering properties of sarcoplasmic reticulum and calcium-induced Ca(2+) release during the quasi-steady level of release in twitch fibers from frog skeletal muscle. Journal of General Physiology 140(4): 403-419, 2012

Myofilament space calcium concentration affects the calcium release rate from the sarcoplasmic reticulum of skinned skeletal muscle fibers of the frog. Biophysical Journal 51(2 Part 2): 104A, 1987

The action of caffeine in promoting ultrastructural damage in frog skeletal muscle fibres. Evidence for the involvement of the calcium-induced release of calcium from the sarcoplasmic reticulum. Naunyn-Schmiedeberg's Archives of Pharmacology 305(2): 159-166, 1978

Two effects of a voltage prepulse on sarcoplasmic reticulum calcium release in frog cut muscle fibers. Biophysical Journal 66(2 Part 2): A340, 1994

Depression of sarcoplasmic reticulum calcium release due to sarcoplasmic reticulum calcium depletion in frog skeletal muscle. Biophysical Journal 49(2 Part 2): 459A, 1986