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Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication



Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication



Nucleic Acids Research 24(8): 1420-1427



We have reconstituted salt-treated SV40 minichromosomes with differentially phosphorylated forms of histone H1 extracted from either G0-, S- or M-phase cells. Sedimentation studies revealed a clear difference between minichromosomes reconstituted with S-phase histone H1 compared with histone H1 from G0- or M-phase cells, indicating that the phosphorylation state of histone H1 has a direct effect on chromatin structure. Using reconstituted minichromosomes as substrate in the SV40 in vitro replication system, we measured a higher replication efficiency for SV40 minichromosomes reconstituted with S-phase histone H1 compared with G0- or M-phase histone H1. These data indicate that the chromatin structure induced by the phosphorylation of histone H1 influences the replication efficiency of SV40 minichromosomes in vitro.

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Accession: 008561520

Download citation: RISBibTeXText

PMID: 8628673

DOI: 10.1093/nar/24.8.1420


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