Endothelin-induced calcium signaling in cultured mouse microglial cells is mediated through ETB receptors

Möller, T.; Kann, O.; Prinz, M.; Kirchhoff, F.; Verkhratsky, A.; Kettenmann, H.

Neuroreport 8(9-10): 2127-2131


ISSN/ISBN: 0959-4965
PMID: 9243597
DOI: 10.1097/00001756-199707070-00008
Accession: 008605354

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Microglial cells are the intrinsic immunocompetent cells of the central nervous system, which are activated by brain tissue damage. In this paper we investigated the ability of endothelins (ETs), which are potent vasoconstrictors, to induce intracellular calcium signals in cultured microglia cells. Both endothelin-1 and endothelin-3 increased intracellular Ca-2+ concentration ((Ca-2+)-i). These (Ca-2+)-i transients were mimicked by BQ3020, an ET-B receptor agonist and blocked by BQ788, a selective ET-B antagonist, respectively. The calcium signals induced by the endothelins persisted in Ca-2+-free media. Transcripts encoding the ET-B receptor were detected in purified microglial cultures and cDNA fragments derived from ET-B receptor mRNA were amplified from 9% of electrophysiologically characterized microglial cells by the use of single-cell RT-PCR.