Enhanced phosphoinositide turnover signalling stimulated by endothelin B-type receptor in endothelial cells from spontaneously hypertensive rats

Yokokawa, K.; Kohno, M.; Minami, M.; Ikeda, M.; Horio, T.; Kano, H.; Hanehira, T.; Yasunari, K.; Takeda, T.

Clinical and Experimental Pharmacology and Physiology. Supplement 22(1): S195-S196

1995


ISSN/ISBN: 0143-9294
PMID: 9072352
DOI: 10.1111/j.1440-1681.1995.tb02878.x
Accession: 008608857

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Abstract
1. Endothelin (ET) B-type (ET-B) receptor-mediated signal transduction was examined after stimulation with ET-3 in cultured aortic endothelial cells (EC) from spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats (8 weeks old). 2. The EC from both rat strains expressed only ET-B receptor mRNA. The receptor densities and affinities, which were nonselective for ET-1, -2, -3 and Sarafotoxin S6c, and mRNA expression were similar in WKY and SHR. 3. The cytosolic Ca-2+ level in the absence of extracellular Ca-2+, inositol 1,4,5-trisphosphate levels, protein kinase C and phospholipase C activities in response to ET-3 were greater in SHR EC than in WKY EC. 4. The 45Ca uptake in response to ET-3, which was blocked by Ni-2+, was smaller in SHR EC than in WKY EC. 5. The 6-keto-PGF-1alpha production was augmented in SHR, though nitric oxide formation after stimulation with ET-3 was similar. 6. These results suggest that ET-B receptor-mediated phosphoinositide turnover signalling is augmented in SHR EC through postreceptor mechanism.

Enhanced phosphoinositide turnover signalling stimulated by endothelin B-type receptor in endothelial cells from spontaneously hypertensive rats