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Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization

Morel, F.; Mercier, S.; Roux, C.; Clavequin, M.C.; Bresson, J.L.

Fertility and Sterility 67(6): 1134-1139

1997


ISSN/ISBN: 0015-0282
PMID: 9176456
DOI: 10.1016/s0015-0282(97)81451-3
Accession: 008625404

Objective: To estimate the mean frequency of aneuploidy levels of chromosomes 8, 15, 18, X, and Y in human sperm, while minimizing the effect of individual factors by analyzing sperm samples from a large set of patients. Design: Prospective randomized analysis of sperm nuclei by fluorescence in situ hybridization. Setting-. University-based laboratory, for reproductive biology. Patient(s): One hundred two patients with a large distribution of sperm parameters, randomly selected from volunteers who had presented seeking a semen analysis. Intervention(s): The sperm samples were prepared for fluorescence in situ hybridization. Main Outcome Measure(s): The disomy frequencies for chromosomes 8, 15, 18, and sex chromosomes were determined using fluorescence in situ hybridization. Result(s): The mean frequencies of disomy for autosomes were 0.18% for chromosome 8, 0.06% for chromosome 15, 0.2% for chromosome 18, and 0.24% for gonosomes (XX, 0.04%; YY, 0.05%; XY, 0.15%). Conclusion(s): This study confirms other previous evaluations on restricted numbers of patients. Our results seem to confirm a relative equiprobability of disomy frequencies concerning the different chromosomal pairs during male meiosis.

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