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Evaluation of sperm preparation methods in in vitro fertilization and embryo transfer for male infertility



Evaluation of sperm preparation methods in in vitro fertilization and embryo transfer for male infertility



Japanese Journal of Fertility & Sterility 38(4): 618-623



Three sperm preparation methods in in vitro fertilization and embryo transfer in male infertility were examined. In the first method, seminal fluid was centrifuged with human tubal fluid and motile sperm was collected by swim up technique. In the second, seminal fluid was centrifuged with 80% Percoll and motile sperm was collected by swim up technique. And in the third approach, sperm was collected in human tubal fluid layer containing pentoxifylline (1 mg/ml) after centrifugation with 80% Percoll. The ratios of total fertilized oocytes to total retrieved oocytes using simple swim-up method, Percoll method, and Percoll-pentoxifylline method, were 9 out of 36 (13%), 23 of 119 (19%), and 54 of 131 (41%), respectively. As for embryo obtained cycles compared with oocyte pick-up cycles, the ratio with the Percoll-pentoxifylline method was higher than with the other two methods. Four pregnancies were obtained from the Percoll-pentoxifylline method. The Percoll-pentoxifylline method was suggested to be useful for sperm preparation in in vitro fertilization and embryo transfer with male infertility.

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Accession: 008636942

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