+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of the genotoxic potential of zinc pyrithione in the Salmonella mutagenicity (Ames) assay, CHO/HGPRT gene mutation assay and mouse micronucleus assay



Evaluation of the genotoxic potential of zinc pyrithione in the Salmonella mutagenicity (Ames) assay, CHO/HGPRT gene mutation assay and mouse micronucleus assay



Journal of Applied Toxicology 13(4): 283-289



The mutagenic potential of zinc pyrithione (Znpt) was evaluated in vitro in the Salmonella/mammalian microsome plate incorporation mutagenicity (Ames) assay and the CHO/HGPRT gene mutation assay. The clastogenic potential of Znpt was evaluated in vivo using the mouse micronucleus test. Znpt was negative in the Ames test in five tester strains in the presence and absence of rat liver microsomal enzymes when assayed at concentrations ranging between 10 and 333 micrograms per plate and between 0.03 and 33 micrograms per plate, respectively. Znpt also produced negative results in the CHO/HGPRT assay. No significant increases in mutant frequencies were seen in the presence and absence of rat liver microsomal enzymes. In each case, the highest concentrations reduced cellular viability by 83% and 85%, respectively. Znpt also did not induce increased frequencies of micronuclei in mouse bone marrow cells when tested at the maximally tolerated dose (MTD) (44 mg kg-1). These data support the conclusion that Znpt lacks genotoxic activity under the conditions of these tests.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008638099

Download citation: RISBibTeXText

PMID: 8376729

DOI: 10.1002/jat.2550130411


Related references

Evaluation of the genotoxic potential of some microbial volatile organic compounds (MVOC) with the comet assay, the micronucleus assay and the HPRT gene mutation assay. Mutation Research, Genetic Toxicology and Environmental Mutagenesis 513(1/2): 143-150, 2002

Evaluation of the genotoxic potential of some microbial volatile organic compounds (MVOC) with the comet assay, the micronucleus assay and the HPRT gene mutation assay. Mutation Research 513(1-2): 143-150, 2001

Mutagenicity tests of diflubenzuron in the micronucleus test in mice, the L5178Y mouse lymphoma forward mutation assay, and the Ames Salmonella reverse mutation test. Mutation Research 66(1): 45-53, 1979

Comparison of genotoxicity of textile dyestuffs in Salmonella mutagenicity assay, in vitro micronucleus assay, and single cell gel/comet assay. Journal of Environmental Pathology, Toxicology and Oncology 23(4): 267-278, 2004

Evaluating the genotoxic effects of workers exposed to lead using micronucleus assay, comet assay and TCR gene mutation test. Toxicology 223(3): 219-226, 2006

Genotoxic evaluation of aspirin eugenol ester using the Ames test and the mouse bone marrow micronucleus assay. Food and Chemical Toxicology 62: 805-809, 2014

Evaluation of the genotoxicity of stevioside and steviol using in vitro mouse lymphoma L5178Y gene mutation assay and in vivo hepatocyte micronucleus assay. Environmental & Molecular Mutagenesis 33(SUPPL 33): 48, 1999

Evaluation of genotoxic effects of lead in pottery-glaze workers using micronucleus assay, alkaline comet assay and DNA diffusion assay. International Archives of Occupational and Environmental Health 85(7): 807-818, 2013

Mutagenicity evaluation of Schistosoma spp. extracts by the umu-test and V79/HGPRT gene mutation assay. Parasitology International 54(1): 29-34, 2005

Mutagenicity of a simulated wastewater effluent in the salmonella ames microsomal gene mutation assay. Environmental Mutagenesis 8(SUPPL 6): 79-80, 1986

Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2. III. Effects in the Salmonella typhimurium/Escherichia coli reversion assay and the mouse lymphoma L5178Y TK+/- forward mutation assay. Mutation Research 241(2): 151-159, 1990

Mutagenicity evaluation of hc blue no. 1 and hc blue no. 2 iii. effects in the salmonella typhimurium and escherichia coli reversion assay and the mouse lymphoma l5178y tk positive negative forward mutation assay. Mutation Research 241(2): 151-160, 1990

The relationship between covalent dna binding and revertant yield in the salmonella mutagenicity ames assay implications for assay specificity. Environmental Mutagenesis 9(SUPPL 8): 27, 1987

Genotoxic activity of antitumor drugs a comparison of ames reverse mutation assay with escherichia coli dna repair assay. Nucleus 28(3): 159-168, 1985

Mutagenicity tests of diflubenzuron in the micro nucleus test in mice the l 5178y mouse lymphoma forward mutation assay the ames salmonella reverse mutation test. Mutation Research 66(1): 45-54, 1979