+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens



Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens



Journal of Clinical Microbiology 36(4): 990-994



CHROMagar Orientation, a new chromogenic medium, was evaluated for the detection and differentiation of gram-positive and gram-negative pathogenic microorganisms in 900 urine samples from hospitalized patients. Performance characteristics of the medium were evaluated in comparison to those of 5% sheep blood and MacConkey agars by direct inoculation of the urine samples on the three media. Four gram-negative and two gram-positive strains as well as one yeast control strain from the American Type Culture Collection were used to ensure quality control. CHROMagar Orientation succeeded in detecting all the urine pathogens that were detected by the reference media, including gram-negative bacilli, staphylococci, streptococci, and yeasts. Colony color and morphology on CHROMagar Orientation accurately differentiated Escherichia coli, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, and Acinetobacter spp. Owing to the similarity in the pigmentation produced by Klebsiella, Enterobacter, and Citrobacter isolates, the medium failed to distinguish among them; however, these isolates were easily recognized as coliforms because of their metallic blue coloration. Staphylococci were clearly perceptible: S. aureus and S. epidermidis grow in regular-size colonies that range from opaque white to yellowish, and S. saprophyticus produces opaque pink colonies. All streptococcus strains, including those from groups B and C, were detected. They grow as undifferentiated flat dry diffused colonies, and additional tests were required for identification. Enterococci were easily discriminated by their strong turquoise pigmentation and their typical growth on the agar's surface. Yeast grow in typical creamy wet convex colonies. The accuracy of antibiotic susceptibility determinations according to standard methods was also tested by picking isolates directly from CHROMagar Orientation. The results showed excellent correlation with those obtained with microorganisms picked from reference media. Owing to the ease in differentiating mixed flora on CHROMagar Orientation, antimicrobic susceptibility tests were performed directly from primary isolates in all cases without the need for subcultures.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008639398

Download citation: RISBibTeXText

PMID: 9542923


Related references

Evaluation of a new chromogenic agar medium for the identification of urinary tract pathogens. Letters In Applied Microbiology. 20(5): 300-302, 1995

Evaluation of Oxoid Chromogenic urinary tract infection medium for detection and presumptive identification of urinary tract pathogens. Abstracts of the General Meeting of the American Society for Microbiology 99: 108, 1999

Evaluation of New bioMérieux Chromogenic CPS Media for Detection of Urinary Tract Pathogens. Journal of Clinical Microbiology 53(8): 2701-2702, 2015

CPS IDA A new chromogenic agar medium for the diagnosis of the most prevalent urinary tract pathogens. Abstracts of the General Meeting of the American Society for Microbiology 94: 536, 1994

Comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens. European Journal of Clinical Microbiology & Infectious Diseases 18(11): 796-803, 1999

Comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens. Clinical Microbiology and Infection 8(10): 641-645, 2002

Comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens. European Journal of Clinical Microbiology and Infectious Diseases 21(4): 283-289, 2002

P168 Comparison of five urine chromogenic agar plates for isolation and identification of urinary tract pathogens. International Journal of Antimicrobial Agents 42: S95-S96, 2013

Clinical evaluation of a novel chromogenic agar dipslide for the diagnosis of urinary tract infections. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 39: 190, 1999

Clinical evaluation of a novel chromogenic agar dipslide for diagnosis of urinary tract infections. European Journal of Clinical Microbiology & Infectious Diseases 19(9): 694-698, 2000

Evaluation of chromogenic UTI media in the presumptive identification of urinary tract pathogens. 2006

Evaluation of two chromogenic media for the isolation and identification of urinary tract pathogens. European Journal of Clinical Microbiology and Infectious Diseases 34(2): 303-308, 2015

Evaluation of BluEcoli (TM): A new chromogenic medium for the isolation and identification of urinary tract pathogens. 2005

Evaluation of a new chromogenic medium, Uriselect 4, for the isolation and identification of urinary tract pathogens. Journal of Clinical Pathology 56(7): 528-531, 2003

Evaluation of a new chromogenic medium for isolation and identification of common urinary tract pathogens. European Journal of Clinical Microbiology and Infectious Diseases 25(2): 108-111, 2006