+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evidence for coronavirus discontinuous transcription

Evidence for coronavirus discontinuous transcription

Journal of Virology 68(4): 2615-2623

Coronavirus subgenomic mRNA possesses a 5'-end leader sequence which is derived from the 5' end of genomic RNA and is linked to the mRNA body sequence. This study examined whether coronavirus transcription involves a discontinuous transcription step; the possibility that a leader sequence from mouse hepatitis virus (MHV) genomic RNA could be used for MHV subgenomic defective interfering (DI) RNA transcription was examined. This was tested by using helper viruses and DI RNAs that were easily distinguishable. MHV JHM variant JHM(2), which synthesizes a subgenomic mRNA encoding the HE gene, and variant JHM(3-9), which does not synthesize this mRNA, were used. An MHV DI RNA, DI(J3-9), was constructed to contain a JHM(3-9)-derived leader sequence and an inserted intergenic region derived from the region preceding the MHV JHM HE gene. DI(J3-9) replicated efficiently in JHM(2)- or JHM(3-9)-infected cells, whereas synthesis of subgenomic DI RNAs was observed only in JHM(2)-infected cells. Sequence analyses demonstrated that the 5' regions of both helper virus genomic RNAs and genomic DI RNAs maintained their original sequences in DI RNA-replicating cells, indicating that the genomic leader sequences derived from JHM(2) functioned for subgenomic DI RNA transcription. Replication and transcription of Dl(J3-9) were observed in cells infected with an MHV A59 strain whose leader sequence was similar to that of JHM(2), except for one nucleotide substitution within the leader sequence. The 5' region of the helper virus genomic RNA and that of the DI RNA were the same as their original structures in virus-infected cells, and the leader sequence of DI(J3-9) subgenomic DI RNA contained the MHV A59-derived leader sequence. The leader sequence of subgenomic DI RNA was derived from that of helper virus: therefore, the genomic leader sequence had a trans-acting property indicative of a discontinuous step in coronavirus transcription.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008641555

Download citation: RISBibTeXText

PMID: 8139040

Related references

Viral replicase gene products suffice for coronavirus discontinuous transcription. Journal of Virology 75(14): 6676-6681, 2001

Discontinuous transcription generates heterogeneity at the leader fusion sites of coronavirus mRNAs. Journal of Virology 62(10): 3870-3873, 1988

Bovine coronavirus 5'-proximal genomic acceptor hotspot for discontinuous transcription is 65 nucleotides wide. Journal of Virology 80(5): 2183-2193, 2006

Structure and functional relevance of a transcription-regulating sequence involved in coronavirus discontinuous RNA synthesis. Journal of Virology 85(10): 4963-4973, 2011

Nucleocapsid phosphorylation and RNA helicase DDX1 recruitment enables coronavirus transition from discontinuous to continuous transcription. Cell Host and Microbe 16(4): 462-472, 2014

Evaluation of the role of heterogeneous nuclear ribonucleoprotein A1 as a host factor in murine coronavirus discontinuous transcription and genome replication. Proceedings of the National Academy of Sciences of the United States of America 98(5): 2717-2722, 2001

Long-distance RNA-RNA interactions in the coronavirus genome form high-order structures promoting discontinuous RNA synthesis during transcription. Journal of Virology 87(1): 177-186, 2013

Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes. Nucleic Acids Research 12(24): 9457-9472, 1984

Evidence of discontinuous transcription in the trypanosomatid Crithidia fasciculata. Journal of Biological Chemistry 262(33): 16192-9, 1987

Analysis of intracellular small RNAs of mouse hepatitis virus: evidence for discontinuous transcription. Virology 156(2): 342-354, 1987

Discontinuous and non-discontinuous subgenomic RNA transcription in a nidovirus. EMBO (European Molecular Biology Organization) Journal 21(23): 6571-6580, 2002

Mechanism of coronavirus transcription: duration of primary transcription initiation activity and effects of subgenomic RNA transcription on RNA replication. Journal of Virology 66(6): 3339-3346, 1992

Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by SARS coronavirus and human coronavirus 229E. Hong Kong Medical Journal 15(Suppl. 9): 23-26, 2009

Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E. Journal of Virology 79(10): 6180-6193, 2005

Leader sequences of murine coronavirus mRNAs can be freely reassorted: evidence for the role of free leader RNA in transcription. Proceedings of the National Academy of Sciences of the United States of America 83(12): 4204-4208, 1986