+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expanding the role of HsEg5 within the mitotic and post-mitotic phases of the cell cycle

Expanding the role of HsEg5 within the mitotic and post-mitotic phases of the cell cycle

Journal of Cell Science 111: 2551-2561

The BimC family of kinesin like proteins are involved in spindle dynamics in a wide variety of organisms. The human member of this family, HsEg5, has been implicated in centrosome separation during prophase/prometaphase and in the organization of in vitro mitotic asters. HsEg5 displays a complex distribution during mitosis, associating with the centrosomes, spindle microtubules, specific regions of the intracellular bridge and a microtubule bundle that forms in association with the post-mitotic migration of the centrosome. In an effort to determine the function of HsEg5 during late mitotic events and refine its proposed function during early mitotic centrosome separation, we microinjected antibodies specific to HsEg5 into HeLa cells during various stages of mitosis. In the presence of HsEg5 antibodies we find that the microtubule arrays responsible for both pre- and post-mitotic centrosome movement never form. Similarly, the microtubule bundle within the intracellular bridge becomes prematurely altered following karyokinesis resulting in the loss of the microtubule array at either end of the bridge. In addition, some pericentrosomal material at the spindle poles becomes fragmented and the distribution of the spindle protein NuMA becomes more concentrated at the minus ends of the spindle microtubules. Our study also provides direct evidence that there is a link between post-mitotic centrosome migration and Golgi complex positioning and reformation following mitosis. We conclude that HsEg5 plays a recurrent role in establishing and/or determining the stability of specific microtubule arrays that form during cell division and that this role may encompass the ability of HsEg5 to influence the distribution of other protein components associated with cell division.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008650866

Download citation: RISBibTeXText

PMID: 9701554

Related references

Variations in the mitotic cycle and in the amount of nuclear DNA in Vicia sativa L. in connexion with some time relationships between the mitotic cycle phases in different botanical groups. Caryologia 26(3): 357-374, 1973

Variations in the mitotic cycle and in the amount of nuclear dNA in Vicia sativa L. displaying some time relationships between the mitotic cycle phases in different botanical groups. Caryologia t 26 (3) 357-374, 1973

Early and late phases of apoptotic injury parallel attempted cell cycle induction in post mitotic neurons. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 198 10, 2002

Post-mitotic role of the cell cycle machinery. Current Opinion in Cell Biology 25(6): 711-716, 2013

Nitrogen deficiency inhibits leaf blade growth in Lolium perenne by increasing cell cycle duration and decreasing mitotic and post-mitotic growth rates. Plant Cell and Environment 31(6): 727-737, 2008

Synthesis of actinomycin-insensitive RNA during the first post-irradiation mitotic cycle, in the synchronously mitotic plasmodia of Physarum polycephalum. Journal of Biosciences (Bangalore): 161-2: 9-19, 1991

Pattern of rna synthesis during the actinomycin insensitive post irradiation mitotic cycle in synchronously mitotic plasmodia of physarum polycephalum. Current Science 59(15): 750-752, 1990

The Drosophila gene morula inhibits mitotic functions in the endo cell cycle and the mitotic cell cycle. Development 124(18): 3543-3553, 1997

Study of the regularities of RNA and protein synthesis in the mitotic cycle of cell cultures using specific inhibitors. II. Progression of mitosis and entry of cells into the S period of the following mitotic cycle. Tsitologiia 13(11): 1332-1340, 1971

FISH-based mitotic and meiotic diakinesis karyotypes of Morus notabilis reveal a chromosomal fusion-fission cycle between mitotic and meiotic phases. Scientific Reports 7: 9573, 2017

Defective control of mitotic and post-mitotic checkpoints in poly(ADP-ribose) polymerase-1(-/-) fibroblasts after mitotic spindle disruption. Cell Cycle 3(3): 335-342, 2004

The role of mitotic kinases in coupling the centrosome cycle with the assembly of the mitotic spindle. Journal of Cell Science 127(Pt 19): 4111-4122, 2014

Role of the Cell Cycle Re-Initiation in DNA Damage Response of Post-Mitotic Cells and Its Implication in the Pathogenesis of Neurodegenerative Diseases. Rejuvenation Research 19(2): 131-139, 2016

Phospho-Ser383-Elk-1 is localized to the mitotic spindles during cell cycle and interacts with mitotic kinase Aurora-A. Cell Biochemistry and Function 31(7): 591-598, 2013

Determination of Cell Cycle Stage and Mitotic Exit Through the Quantification of the Protein Levels of Known Mitotic Regulators. Methods in Molecular Biology 1505: 45-57, 2017