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Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; Influence of growth conditions and a recombinant positive regulatory gene



Expression of colonization factor antigen I fimbriae by enterotoxigenic Escherichia coli; Influence of growth conditions and a recombinant positive regulatory gene



Apmis. 105(3): 247-254



Enterotoxigenic Escherichia coli (ETEC) may spontaneously lose the positive regulatory cfaR gene and thereby the capacity to express colonization factor antigen I (CFA/I). A recombinant plasmid harbouring the cfaR gene was transformed into cfaR-negative mutant ETEC strains. CFA/I expression of wild-type and cfaR-transformed ETEC cultivated in different liquid media was quantified. At 37 degrees C, a high level of CFA/I expression from wild-type and cfaR-transformed strains was observed after growth in CFA broth. Transformation enhanced CFA/I expression only marginally. The transformant cultures showed a considerable variation in CFA/I expression which was paralleled by the proportion of individual bacteria producing CFA/I. This heterogeneity could be explained by a variable tendency to structural CFA/I gene loss among individual cfaR-transformed bacteria.

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Accession: 008659986

Download citation: RISBibTeXText

PMID: 9137521

DOI: 10.1111/j.1699-0463.1997.tb00565.x



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