EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Expression of the sodium, calcium exchanger in a diverse tissues: A study using the cloned human cardiac sodium, calcium exchanger


Expression of the sodium, calcium exchanger in a diverse tissues: A study using the cloned human cardiac sodium, calcium exchanger



American Journal of Physiology 263(6 Part 1): C1241-C1249



ISSN/ISBN: 0363-6143

In many cells including cardiac myocytes, cytoplasmic Ca is importantly controlled by the plasmalemmal Na-Ca exchanger (3, 8). The tissue diversity and differences in cellular environment raise the question whether the same exchanger is found in all tissues. Recent experiments using rod cells hasve demonstrated that at least two forms of Na-dependent Ca transport exist. We have examined this issue in various rat and human tissues using the cloned human cardiac Na-Ca exchanger cDNA. Northern blot analysis in these two species show that the major transcript of the Na-Ca exchanger is 7.2 kilobases in heart, brain, kidney, liver, pancreas, skeletal muscle, placenta, and lung. Furthermore, ribonuclease protection analysis in rats shows conservation of the 348-base pair segment tested in heart, brain, kidney, skeletal muscle, and liver. Additionally, southern blot analysis suggest that a single gene encodes this Na-Ca exchanger. Finally, we show that the clone used to generate our pirobes encodes a completely functional Na-Ca exchanger. With the use of COS cells and 293 cells transfected with the cloned human cardiac Na-Ca exchanger, we tested the Ca transport properties of the Na-Ca exchanger, the voltage dependence of the Na-Ca exchanger, as well as the Na dependence of the transport function of the Na-Ca exhanger. We conclude that the cardiac form of the Na-Ca exchanger is completely functional when the cDNA is expressed in mammalian cell lines, and, furthermore, this "cardiac" form of the Na-Ca exchanger is naturally expressed in all human and rat tissues tested (but at varying levels).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008664917

Download citation: RISBibTeXText

Related references

Expression of the Na-Ca exchanger in diverse tissues: a study using the cloned human cardiac Na-Ca exchanger. American Journal of Physiology 263(6 Pt 1): C1241-C1249, 1992

Charge movement during sodium translocation by native and cloned cardiac sodium calcium exchanger. Nature (London) 352(6337): 715-718, 1991

Functional distinction of the exchanger inhibitor peptide binding- and the calcium-activation site of the cardiac sodium-calcium exchanger. Biophysical Journal 86(1): 450a-451a, 2004

Cardiac sodium-calcium exchanger is regulated by allosteric calcium and exchanger inhibitory peptide at distinct sites. Circulation Research 96(1): 91-99, 2004

Calcium fluxes in human trophoblast (BeWo) cells: calcium channels, calcium-ATPase, and sodium-calcium exchanger expression. Molecular Reproduction and Development 64(2): 189-198, 2003

Regulation of sodium-calcium exchange by cytosolic calcium in CHO cells transfected with the bovine cardiac sodium-calcium exchanger. Biophysical Journal 66(2 Part 2): A332, 1994

The human cardiac sodium calcium exchanger cloning sequencing and expression. Biophysical Journal 61(2 Part 2): A387, 1992

Increased sodium/calcium exchanger activity enhances beta-adrenergic-mediated increase in heart rate: Whole-heart study in a homozygous sodium/calcium exchanger overexpressor mouse model. Heart Rhythm 14(8): 1247-1253, 2017

ATP-dependent regulation of sodium-calcium exchange activity in CHO cells transfected with the bovine cardiac sodium-calcium exchanger. Biophysical Journal 66(2 Part 2): A332, 1994

Regulation of sodium-dependent barium influx by cytosolic calcium in transfected CHO cells expressing the cardiac sodium-calcium exchanger. Biophysical Journal 72(2 Part 2): A64, 1997

Antibody to the cardiac sodium-calcium exchanger inhibits sodium-calcium exchange current measured in giant excised patches. Biophysical Journal 64(2 Part 2): A397, 1993

ATP-dependent regulation of sodium-calcium exchange in Chinese hamster ovary cells transfected with the bovine cardiac sodium-calcium exchanger. Journal of Biological Chemistry 270(16): 9137-9146, 1995

Determination of immunoreactive sodium calcium exchanger and sodium dependent calcium uptake in cardiac sarcolemma from fetal newborn immature and adult rabbits. Circulation 84(4 Suppl 2): II188, 1991

Integrin-mediated membrane blebbing is dependent on sodium-proton exchanger 1 and sodium-calcium exchanger 1 activity. Journal of Biological Chemistry 287(13): 10316-10324, 2012

Possible charge movement of extracellular sodium binding by sodium potassium pump and cardiac sodium calcium exchanger in giant patches. Biophysical Journal 61(2 Part 2): A390, 1992