+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

External beam radiation enhances antibody mediated radiocytotoxicity in human glioma cells in vitro



External beam radiation enhances antibody mediated radiocytotoxicity in human glioma cells in vitro



Anticancer Research 17(3b): 1797-1802



Enhanced accumulation of monoclonal antibodies in tumor tissue has been observed as a result of external beam irradiation (EBR). This effect was mainly attributed to increased vascular leakage due to unspecific radiation damage of vascular endothelial cells. The aim of this study was to investigate the effects of EBR on expression and antibody-binding of epidermal growth-factor receptor (EGF-R) in human glioma cells in-vitro. High-grade glioma cells were irradiated with conventional x-rays (0-3600 Rad) and surface binding, internalization and radiocytotoxicity of 125I-labeled monoclonal antibody (MAb) 425, specific for human EGF-R, was tested. EBR showed a short-term dose and time dependent increase of specific MAb 425 binding and internalization in receptor positive cell lines U87-MG and A1207. This effect was probably due to a mitotic block and an increase in cellular volume. Combination of EBR and 125I-425 showed additive effects on cell vitality/survival and was more pronounced in contact inhibited cells as compared to cells growing in a log-phase. We assume that cells exposed to 125I-labeled MAb 425 are only able to accumulate a critical number of DNA double-strand breaks when the doubling-time is prolonged e.g. under contact-inhibition or radiation induced mitotic blockade. We conclude that EBR has no negative effects on EGF-R expression, MAb-binding and internalization. The combination of EBR and 125I-MAb 425 enhances cytotoxic efficacy and thus supports adjuvant use in the clinical management of high-grade glioma.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008666819

Download citation: RISBibTeXText

PMID: 9179236


Related references

External beam radiotherapy of recurrent glioma: radiation tolerance of the human brain. Cancers 4(2): 379-399, 2012

Quantitative comparison of radiolabeled antibody therapy and external beam radiotherapy in the treatment of human glioma xenografts. International Journal of Radiation Oncology Biology Physics 24(1): 111-117, 1992

L-boronophenylalanine-mediated boron neutron capture therapy for malignant glioma progressing after external beam radiation therapy: a Phase I study. International Journal of Radiation Oncology Biology Physics 80(2): 369-376, 2011

Accumulation of CD133-positive glioma cells after high-dose irradiation by Gamma Knife surgery plus external beam radiation. Journal of Neurosurgery 113(2): 310-318, 2010

Low-dose radiation enhances survivin-mediated virotherapy against malignant glioma stem cells. Cancer Research 68(14): 5778-5784, 2008

Silencing of HIF-1α enhances the radiation sensitivity of human glioma growth in vitro and in vivo. Neuropharmacology 89: 168-174, 2015

Histologically Proven Radiation-Induced Brainstem Glioma 93 Months After External Beam Radiotherapy for Pituitary Macroadenoma: Radiation Treatment Dose and Volume Correlation. Journal of Neuroimaging 25(4): 674-676, 2016

Inactivation of human osteosarcoma cells in vitro by 211At-TP-3 monoclonal antibody: comparison with astatine-211-labeled bovine serum albumin, free astatine-211 and external-beam X rays. Radiation Research 139(2): 178-184, 1994

Augmentation of anti-Fas antibody-mediated apoptosis on human glioma cells by liposomes associated with the antibody. JournalofNeuro-Oncology35(1):7, 1997

External beam radiation dose escalation for high grade glioma. Cochrane Database of Systematic Reviews 2016(8): Cd011475, 2016

A randomized trial comparing iridium implant and external beam radiation vs external beam radiation in node negative locally advanced cancer of the prostate Preliminary report. Clinical & Investigative Medicine (Suppl. ): S95, 1998

Hypofractionated external-beam radiation therapy (HEBRT) versus conventional external-beam radiation (CEBRT) in patients with localized prostate cancer: a systematic review and meta-analysis. Core Evidence 8: 1-13, 2013

External beam radiation of tumors alters phenotype of tumor cells to render them susceptible to vaccine-mediated T-cell killing. Cancer Research 64(12): 4328-4337, 2004

Vitamin D(3) enhances ATRA-mediated neurosteroid biosynthesis in human glioma GI-1 cells. Journal of Biochemistry 152(3): 285-292, 2012

Dihydroartemisinin enhances radiosensitivity of human glioma cells in vitro. Journal of Cancer Research and Clinical Oncology 132(2): 129-135, 2005