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Extracellular adenosine-5'-triphosphate increases intracellular calcium concentration of the rabbit eye suprachoroid via activation of the intracellular signal transduction



Extracellular adenosine-5'-triphosphate increases intracellular calcium concentration of the rabbit eye suprachoroid via activation of the intracellular signal transduction



Nippon Ganka Gakkai Zasshi 98(1): 69-74



Intracellular calcium concentration ((Ca-2+)-i) of the rabbit eye suprachoroid was measured with microfluorophotometry using fura-2. Adenosine-5'-triphosphate (ATP) 1 apprx 100 mu-M, added to a perfusing solution, elicited an increase in (Ca-2+)-i, of the suprachoroid. This action of ATP was inhibited when preparations were perfused with a nominally zero Ca-2+ Krebs solution or a high magnesium Krebs solution. The (Ca-2+)-i-increasing action of ATP was attenuated either by lowering temperature or by a phospholipase C inhibitor, compound 48/80. These results indicate that extracellular Ca-2+ is necessary to the ATP action and also suggest that the ATP action may be mediated by the intracellular signal transduction which exerts a Ca-2+-release from intracellular Ca-2+-store sites. The potency order of ATP-related purine nucleotides was ATP gtoreq ATP-gamma-S gt adenosine-5'-diphosphate (ADP). Adenosine-5'-monophosphate (AMP) and adenosine were not effective. It is concluded that P-2-purinoceptors exist on the surface of the suprachoroid and are involved in mechanisms underlying the ATP-induced increase in (Ca-2+)-i of the suprachoroid.

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Accession: 008667455

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PMID: 8109448


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