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Factors affecting sperm yield during swim-up

, : Factors affecting sperm yield during swim-up. Journal Of Assisted Reproduction & Genetics. 10(2): 145-150

Purpose: The migration of sperm (expressed as the percentage of the total motile cells in the ejaculate aliquot) from seminal plasma into overlying medium (swim-up) was increased with time, temperature, and increasing the surface area of contact between the two phases by increasing the single of tube inclination. Results: The proportion of sperm migrating into the medium was relatively constant with varying sperm count. Stimulation of the progressive motility of spermatozoa in vitro using a motility enhancing drug (2-deoxyadenosine) increased sperm migration initially but no more than the maximum reached by controls after extended incubation. Conclusion: Sperm yield from normozoospermic men and men with borderline asthenoteratozoospermia represented approximately 40 and 15% of the total motile cells in the ejaculate aliquots, respectively. In general, in both groups spermatozoa in the swim-up fraction exhibited superior motility, vitality, and morphology compared to the original ejaculates. Tail abnormalities were reduced more than head abnormalities and subjects with borderline sperm qualities exhibited less dramatic improvements in quality than the normozoospermic individuals.

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Mallin, Ma, 1986: The feeding ecology of the blue tilapia (T. aurea) in a North Carolina reservoir. Lake and Reservoir Management 2: 323-326

Balsam William; Ji Junfeng; Chen Jun, 2004: Climatic interpretation of the Luochuan and Lingtai loess sections, China, based on changing iron oxide mineralogy and magnetic susceptibility. Hematite and goethite, estimated from diffuse reflectance spectra, and magnetic susceptibility (MS) have been determined from both the Luochuan and Lingtai loess sections of the Loess Plateau of central China. Changing concentrations of these iron...

Belmares, H; Castillo, Je; Barrea, A., 1979: Natural hard fibers of the North American continent. Statistical correlations of physical and mechanical properties of lechuguilla (Agave lechuguilla) fibers. Textile research journal 49(11): 619-622

Casuccio, C., 1962: An introduction to the study of osteoporosis (biochemical and biophysical research in bone ageing). Changes in morphology and staining properties during the ageing of bone are described from study of 12th thoracic and 1st lumbar vertebrae from 8 subjects under 30 years of age, 16 aged 30 to 50, 29 aged 50 to 65 and 50 aged 65 to 93 years. The na...

Zink, T. L.; Gaskins, C. T.; Hillers, J. K., 1979: Effect of season of calving, weaning age, and method of breeding on economic returns of beef production systems. Computer modelling was used to examine the effects of spring, autumn and winter calving seasons, weaning at 60, 120, 180 and 240 days of age and breeding by natural service (NS) or artificial insemination (AI) on the profitability of cow-calf prod...

Flake, R. H.; Lennington, R. K., 1977: Genetic distances utilising electrophoretic mobility data. A new definition of genetic distance between populations is formulated. For a given population, the frequencies of the genes at a locus are ordered in a vector (Mij), according to the electrophoretic mobility of the products that the genes control...

Park, H. J.; Lyons, J. C.; Ohtsubo, T.; Song, C. W., 1999: Acidic environment causes apoptosis by increasing caspase activity. An exposure of HL-60 human promyelocytic leukaemia cells to acidic media with pH 6.2-6.6 caused an up-regulation of Bax protein expression within 2 h, which lasted for longer than 6 h. On the other hand, the apoptosis, as judged from PARP cleavage...

Andjus, L., 1990: Finding of yucca thrips Bagnalliella yuccae Hinds in Yugoslavia. Unknown

Dr. K. Fehske, A. Steinert, H.-J. Eichhorn, 2013: Intraoperative biologische Augmentation an Ligamenten. Als Schl ssel zur optimalen operativen Versorgung von ligament ren Verletzungen z hlt mittlerweile neben der Ber cksichtigung der exakten Anatomie auch die der Biologie. Nach frischen Bandverletzungen kommt es bei extraartikul ren Ligamenten (z. B...

MacDonald, David., 1995: European mammals: evolution and behaviour. Unknown