EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Fed-batch production of Thermomonospora fusca endoglucanase by recombinant Streptomyces lividans



Fed-batch production of Thermomonospora fusca endoglucanase by recombinant Streptomyces lividans



Biotechnology & Bioengineering. 60(1): 70-76,. 5



The factors affecting the production of a Thermomonospora fusca endoglucanase by a recombinant Streptomyces lividans strain were studied in a fermentor with glucose addition controlled by a pH-stat. The recombinant plasmid was stable for 35 generations with constant endoglucanase productivity. Glucose and peptone were used as the carbon and nitrogen sources. Addition of Tween-80 increased endoglucanase production twofold. A significant decrease in endoglucanase production was observed at low aeration. During fed-batch cultivation, pulse feeding (6 g/L) of a glucose-ammonium sulfate solution was optimal for endoglucanase production. With higher concentrations of glucose (15 g/L), a significant amount of organic acid, including acetic acid, was produced, which inhibited cell growth and endoglucanase production. Under optimum conditions, 1.7 U/mL of endoglucanase were produced.

Accession: 008680365

Download citation: RISBibTeXText

DOI: 10.1002/(sici)1097-0290(19981005)60:1<70::aid-bit8>3.0.co;2-y

Download PDF Full Text: Fed-batch production of Thermomonospora fusca endoglucanase by recombinant Streptomyces lividans



Related references

Fed-batch production of thermomonospora fusca endoglucanase by recombinant streptomyces lividans. Biotechnology and Bioengineering 60(1): 70-76, 1999

DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca. Applied and Environmental Microbiology 59(9): 3032-3043, 1993

Cloning of the Thermomonospora fusca Endoglucanase E2 Gene in Streptomyces lividans: Affinity Purification and Functional Domains of the Cloned Gene Product. Applied and Environmental Microbiology 54(10): 2521-2526, 1988

Expression of a Thermomonospora fusca Cellulase Gene in Streptomyces lividans and Bacillus subtilis. Applied and Environmental Microbiology 53(7): 1470-1475, 1987

Cloning of Thermomonospora fusca cellulase genes in Escherichia coli and Streptomyces lividans. Adolph, Kenneth W Methods in Molecular Genetics; Microbial gene techniques 367-374, 1995

Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in Streptomyces lividans. Applied and Environmental Microbiology 62(11): 4256-4259, 1996

Cloning of a Thermomonospora fusca xylanase gene and its expression in Escherichia coli and Streptomyces lividans. Journal of Bacteriology 171(6): 2963-2969, 1989

Production of thermostable xylanases in batch and continuous culture by thermomonospora fusca kw 3. Applied Microbiology & Biotechnology 37(4): 416-419, 1992

Dimerization of Thermomonospora fusca b-1,4-endoglucanase E2. Biochemistry (American Chemical Society) 32: 46-50, 1993

DNA sequences of three b-1,4-endoglucanase genes from Thermomonospora fusca. Journal of Bacteriology 173(11): 97-407, 1991

Dimerization of Thermomonospora fusca beta-1,4-endoglucanase E2. Biochemistry. 32(32): 8146-8150, 1993

Regulation of b-1,4-endoglucanase synthesis in Thermomonospora fusca. Applied and Environmental Microbiology 53: 52-7, 1987

DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca. Journal of Bacteriology 173(11): 3397-3407, 1991

Regulation of beta-1,4-Endoglucanase Synthesis in Thermomonospora fusca. Applied and Environmental Microbiology 53(6): 1352-1357, 1987