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Feline beta-lactoglobulins I, II and III, and canine beta-lactoglobulins I and II: Amino acid sequences provide evidence for the existence of more than one gene for beta-lactoglobulin in the cat and dog

Feline beta-lactoglobulins I, II and III, and canine beta-lactoglobulins I and II: Amino acid sequences provide evidence for the existence of more than one gene for beta-lactoglobulin in the cat and dog

Protein Sequences & Data Analysis 5(5): 201-205

The complete amino acid sequences of two feline and two canine beta-lactoglobulins (BLGs) are presented in this report. The proteins were purified from milks obtained from several different individuals. Both feline BLGs I and III have a total of 162 amino acids and the canine BLGs I and II have 161 amino acids, a deletion occurring at position 151. The sequence of feline BLG II has been reported elsewhere (1). Labelling experiments show that feline beta-lactoglobulins I and III have a cysteine at position 121, while cystines at 66 and 160 plus 106 and 119 are present and presumably they bridge to give the two disulphide bonds conserved in all other BLGs. Although there is significant positional identity between the sequences of the three feline BLGs when pairwise comparisons of the BLGs are examined, between 16% and 33% of the substitutions represent double base changes in the nucleotide codons for the amino acids. These observations and previously presented electrophoretic data (2) suggest that there are three BLG genes in cats. The evidence for two BLG genes in the dog is less convincing as only 2 of the 15 amino acid substitutions between canine BLGs I and II represent double base changes. In the cat BLGs the majority of the amino acid substitutions occur in the region of the protein encoded by exons III and IV. In the dog BLGs most substitutions are in the segment of the protein that would be encoded by exon III.

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