+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM

Fertilization in vitro of mouse ova from inbred and outbred strains: complete preimplantation embryo development in glucose-supplemented KSOM

Biology of Reproduction 53(2): 431-437

ISSN/ISBN: 0006-3363

PMID: 7492697

DOI: 10.1095/biolreprod53.2.431

A new medium derived from the use of sequential simplex optimization methods (SOM) that overcomes the block to development beyond two cells in vitro in embryos of the CF1-cultured strain of mouse has recently been described. Contrary to previous reports, glucose was shown to have no significant inhibiting effect on embryo development to the blastocyst stage in SOM. A modification of SOM, designated KSOM, with an increased concentration of Na+ (95 mM) and K+ (2.5 mM), which has been described elsewhere, also supports growth beyond the two-cell block. KSOM produces a higher rate of compaction, a larger yield of blastocysts, and an increased rate of cell division of the trophoblast cells. We have reexamined the glucose effect by varying the concentration of glucose (either 0.2 mM or 5.56 mM) in KSOM and determined the ability of these media to support preimplantation development of CF1 female times B6D2F1 male zygotes through the blastocyst stage. Glucose is shown to have no significant inhibiting effect on development to the blastocyst stage. The yield of blastocysts is typically 85%-90%. A modification of KSOM derived from this study, designated modified KSOM, with an increased concentration of glucose (5.56 mM) and supplemented with 4 mg ml-1 BSA is now shown to support high rates of fertilization in vitro of CF1 ova with hybrid B6D2F1/CrlBR sperm and subsequent development of zygotes beyond the two-cell stage to blastocysts in high yield. A single medium, modified KSOM, thus supports both fertilization in vitro of CF1 ova and complete preimplantation embryo development without the need for a change of medium to overcome the two-cell block. The overall yield of blastocysts from CF1 ova is 80%-85%. Embryo transfer experiments at the pronuclear, two-cell, and early blastocyst stages gave high rates of implantation and live fetuses from in vitro-fertilized CF1 oocytes. The general utility of modified KSOM for the fertilization of mouse ova in vitro is confirmed by obtaining high rates of fertilization in vitro and blastocyst development of two F1 hybrid strains: B6D2F1/J(C57BL/6J female times DBA/2J male ) and B6CBAF1/J(C57BL/6J female times CBA/J male ).

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 008682812

Download citation: RISBibTeXText

Related references

Differential expression of G6PDH mRNA transcripts during Kunming mouse preimplantation embryo development in CZB and KSOM. Chinese Journal of Veterinary Science 22(4): 359-362, 2002

Mouse oocyte maturation fertilization and embryo cultivation in vitro in inbred strains with stage specific blocks during early embryogenesis. Experientia 46(ABSTR): A14, 1990

Fertilization and development in vitro of mouse eggs from inbred strains and F1 hybrids. Japanese Journal of Animal Reproduction 24(1): 19-22, 1978

Influence of glucose on in vitro development rate of Mus domesticus domesticus 1-cell stage embryos in HEPES supplemented KSOM or HTF media. Acta Scientiae Veterinariae 30(2): 79-85, 2002

Fertilization and preimplantation development in vitro of mouse eggs obtained following stimulation with different doses of pregnant mare serum: a comparison of the responses in two strains. Differentiation; Research in Biological Diversity 9(1-2): 29-35, 1977

In vitro maturation and in vitro fertilization of mouse oocytes and preimplantation embryo culture. Methods in Molecular Biology 1150: 191-199, 2014

Optimization of the system for embryogenetic studies on inbred strains of mice. 3. Conditions of hormonal stimulation of females and in vitro culture of preimplantation embryos of ICR, CBA/Lac and C57Bl/6j mouse strains. Biopolimery i Kletka 13(3): 232-238, 1997

Influence of in vitro cultivation and in vitro fertilization on the incidence of chromosomal aberrations in the preimplantation embryo of the mouse. Zuchthygiene 19(3): 123, 1984

Superovulation, in vitro fertilization (IVF) and in vitro development (IVD) protocols for inbred BALB/cJ mice in comparison with outbred NMRI mice. Reproductive Medicine and Biology 11(4): 185-192, 2012

In vitro fertilization affects growth and glucose metabolism in a sex-specific manner in an outbred mouse model. Biology of Reproduction 90(4): 80, 2014

Preimplantation development of the primate embryo after in vitro fertilization. Journal of Experimental Zoology 228(2): 215-221, 1983

Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. Journal of Reproduction and Fertility 96(1): 219-231, 1992

Osteopontin is expressed in the oviduct and promotes fertilization and preimplantation embryo development of mouse. Zygote 23(4): 622-630, 2015

Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Molecular Reproduction and Development 41(2): 232-238, 1995

Role of glucose in mouse preimplantation embryo development. Molecular Reproduction and Development 40(4): 436-443, 1995