+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Flow cytometric and functional analyses of central nervous system-infiltrating cells in SJL/J mice with Theiler's virus-induced demyelinating disease. Evidence for a CD4+ T cell-mediated pathology



Flow cytometric and functional analyses of central nervous system-infiltrating cells in SJL/J mice with Theiler's virus-induced demyelinating disease. Evidence for a CD4+ T cell-mediated pathology



Journal of Immunology 156(10): 4050-4058



Theiler's murine encephalomyelitis viruses (TMEVs) are endemic enteric pathogens of mice that cause immune-mediated, chronic, progressive, central nervous system (CNS) demyelinating disease in susceptible strains. Analysis of T cell phenotype and functional state from TMEV-infected SJL/J mice by flow cytometry reveals that 13.5 to 25% of the CD4+ T cells in the CNS express high affinity IL-2R, a marker of recent T cell activation, whereas splenic levels of CD4+IL-2R+ T cells generally range between 2 and 8.5%. In contrast, very few CD8+ T cells (<1-2%) from either site express IL-2R. From days 20 to 119 postinfection, the percentage of CD4+IL-2R+ T cells increases gradually in the CNS, but varies little in the spleen. CD4+ T cells isolated from the spinal cord of infected mice proliferate in vitro in response to viral Ag. Similar T cell phenotypes were found in experimental autoimmune encephalomyelitis, an established model of CD4+ T cell-mediated demyelination. In addition, most CD4+ and CD8+ T cells in CNS isolates from TMEV-infected mice are CD44+, indicating that prior activation may be required to traffic through and/or be retained in the CNS. Finally, TCR V beta region usage as well as IL-2R expression by individual V beta region subsets are heterogeneous in both the CNS and spleen. These results are consistent with a model in which a polyclonal population of TMEV-specific, CD4+ Th1 cells plays a major effector role in the demyelinating process.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008692518

Download citation: RISBibTeXText

PMID: 8621948


Related references

The majority of infiltrating CD8+ T cells in the central nervous system of susceptible SJL/J mice infected with Theiler's virus are virus specific and fully functional. Journal of Virology 76(13): 6577-6585, 2002

Flow cytometric analysis of cells infiltrating the CNS of SJL/J mice with Theilers murine encephalomyelitis virus-induced demyelinating disease. Journal of Immunology 150(8 Part 2): 27A, 1993

Central nervous system chemokine expression during Theiler's virus-induced demyelinating disease. Journal of Neurovirology 5(6): 635-642, 1999

Viral hyperinfection of the central nervous system and high mortality after hematopoietic stem cell transplantation for treatment of Theiler's murine encephalomyelitis virus-induced demyelinating disease. Blood 94(8): 2915-2922, 1999

Apoptosis of infiltrating T cells in the central nervous system of mice infected with Theiler's murine encephalomyelitis virus. Virology 315(1): 0-23, 2003

Identification of Theiler's virus infected cells in the central nervous system of the mouse during demyelinating disease. Microbial Pathogenesis 3(5): 319-326, 1987

Fibrin deposition in the central nervous system correlates with the degree of Theiler's murine encephalomyelitis virus-induced demyelinating disease. Journal of Neuroimmunology 77(2): 185-194, 1997

Class ii restricted t cell responses in theiler's murine encephalomyelitis virus tmev induced demyelinating disease ii. survey of host immune responses and central nervous system virus titers in inbred mouse strains. Microbial Pathogenesis 3(5): 327-338, 1987

Theiler's virus RNA and protein synthesis in the central nervous system of demyelinating mice. Virology 144(1): 290-294, 1985

Expression and potential role of inducible nitric oxide synthase in the central nervous system of Theiler's murine encephalomyelitis virus-induced demyelinating disease. Cellular Immunology 194(2): 186-193, 1999

Theiler's virus-induced central nervous system disease in mice. Ucla Forum in Medical Sciences 1976(19): 263-277, 1976

A protein clinical for a Theiler's virus-induced immune system-mediated demyelinating disease has a cell type-specific antiapoptotic effect and a key role in virus persistence. Journal of Virology 72(11): 8605-8612, 1998

CCL2 transgene expression in the central nervous system directs diffuse infiltration of CD45(high)CD11b(+) monocytes and enhanced Theiler's murine encephalomyelitis virus-induced demyelinating disease. Journal of Neurovirology 9(6): 623-636, 2003

Characterization of and functional antigen presentation by central nervous system mononuclear cells from mice infected with Theiler's murine encephalomyelitis virus. Journal of Virology 72(10): 7762-7771, 1998

B-lymphocyte requirement for vaccine-mediated protection from Theiler's murine encephalomyelitis virus-induced central nervous system disease. Journal of Virology 69(8): 5152-5155, 1995