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Functional coupling of Gi subtypes with GABA-B receptor/adenylyl cyclase system: Analysis using a reconstituted system with purified GTP-binding protein from bovine cerebral cortex



Functional coupling of Gi subtypes with GABA-B receptor/adenylyl cyclase system: Analysis using a reconstituted system with purified GTP-binding protein from bovine cerebral cortex



Neurochemistry International 31(1): 21-25



A single molecular species of GTP-binding protein (G protein) has been purified from the bovine cerebral cortex. The immunoblot analysis indicated that the isolated G protein might be Gi-1 or Gi-2 but not Go, since it was reacted by specific antibodies, anti-Gi-alpha-1-2 and anti-Gi-alpha-1-3, but not anti-Go-alpha. When the Gi protein was reconstituted into phospholipid vesicles with partially purified GABA-B receptor and adenylyl cyclase, the stimulation of GABA-B receptor by its agonists induced the inhibition of forskolin-stimulated cAMP accumulation. This GABA-induced inhibition was abolished by CGP 55845A, an antagonist of GABA-B receptor. These results suggest that a Gi subtype, which was suggested to correspond to G-i, or Gi-2 may be functionally coupled with GABA-B receptor/adenylyl cyclase system.

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Accession: 008709175

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PMID: 9185160


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