Functional expression of a recombinant unitary glutamate receptor from Xenopus, which contains N-methyl-D-aspartate (NMDA) and non-NMDA receptor subunits
Soloviev, M.M.; Brierley, M.J.; Shao, Z.Y.; Mellor, I.R.; Volkova, T.M.; Kamboj, R.; Ishimaru, H.; Sudan, H.; Harris, J.; Foldes, R.L.; Grishin, E.V.; Usherwood, P.N.; Barnard, E.A.
Journal of Biological Chemistry 271(51): 32572-32579
ISSN/ISBN: 0021-9258 PMID: 8955083 DOI: 10.1074/jbc.271.51.32572
A cDNA encoding a 100-kDa subunit (XenNR1) of the N-methyl-D-aspartate (NMDA) glutamate receptor type has been cloned from Xenopus central nervous system. When XenNR1 is coexpressed in a mammalian cell line with a recently cloned 51-kDa non-NMDA receptor subunit (XenU1), also from Xenopus, it forms a functional unitary receptor exhibiting the pharmacological properties characteristic of both NMDA and non-NMDA receptors.