+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Further analysis of interleukin-2 receptor subunit expression on the different human peripheral blood mononuclear cell subsets



Further analysis of interleukin-2 receptor subunit expression on the different human peripheral blood mononuclear cell subsets



Blood 91(1): 165-172



We have investigated the expression of the three components of the interleukin-2 receptor (IL-2Ralpha, IL-2Rbeta, and IL-2Rgamma) on the surface of the various peripheral blood mononuclear cell (PBMC) subsets by flow cytometry analysis. The PBMC were immediately isolated (ficoll) from blood collected on heparin as anticoagulant. The three IL-2R components are absent or only marginally detectable on CD4 T lymphocytes. No expression of the IL-2R chains is found for the B lymphocytes. In most donors, the three chains are not detectable on CD8 T lymphocytes, but for a few of them, IL-2Rbeta or IL-2Rgamma are clearly expressed. CD56 high (IL-2Ralpha+) and CD56 low (IL-2Ralpha-) natural killer INK) cells express IL-2Rbeta, but not IL-2Rgamma. IL-2Rgamma is expressed by monocytes of all donors although with variable intensity. When blood is collected on other anticoagulants or when cells are isolated 1 day after collection, IL-2Ralpha, IL-2Rbeta, and IL-2Rgamma are largely expressed on the surface of most PBMC. This observation provides a possible explanation for divergent data previously reported on IL-2R expression. Finally, we show that IL-2Rgamma, which is not detectable on the cell surface of lymphocytes, is nevertheless expressed and stored as an intracellular component. This result is in agreement with the constitutive expression of the IL-2Rgamma gene and suggests a specific regulatory mechanism for IL-2Rgamma membrane translocation.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008712782

Download citation: RISBibTeXText

PMID: 9414281


Related references

Differential expression of somatostatin receptor subtypes in human peripheral blood mononuclear cell subsets. European Journal of Endocrinology 150(4): 565-577, 2004

Analysis of perforin expression in human peripheral blood lymphocytes CD 56+ natural killer cell subsets and its induction by interleukin-2. Immunological Investigations. 24(3): 499-507, 1995

Molecular analysis of human interleukin-9 receptor transcripts in peripheral blood mononuclear cells. Identification of a splice variant encoding for a nonfunctional cell surface receptor. Journal of Biological Chemistry 273(37): 24016-24024, 1998

Isoprinosine potentiation of human peripheral blood mononuclear cell response to mitogens: kinetics and effect on expression of the IL-2 receptor and the activity of interleukin 2. Journal of Clinical and Laboratory Immunology 27(2): 91-96, 1988

Regulation of interleukin-12 receptor beta1 chain expression and interleukin-12 binding by human peripheral blood mononuclear cells. European Journal of Immunology 27(1): 147-154, 1997

Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid -oxidation: functional role of peroxisome proliferatoractivated receptor in human peripheral blood mononuclear cells. The American Journal of Clinical Nutrition 86(5): 1515-1523, 2007

Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid o-oxidation: functional role of peroxisome proliferator-activated receptor l in human peripheral blood mononuclear cells. American journal of clinical nutrition AJN 86(5): 1515-1523, 2007

Inhibition of interleukin-1 beta mRNA expression and interleukin-1 alpha and beta secretion by a specific human recombinant interleukin-1 receptor antagonist in human peripheral blood mononuclear cells. Immunology 77(2): 245-250, 1992

Histamine and histamine-receptor antagonists modify gene expression and biosynthesis of interferon gamma in peripheral human blood mononuclear cells and in CD19-depleted cell subsets. Immunology Letters 70(2): 95-99, 1999

The effect of anti-CD3 monoclonal antibody stimulation on the expression o p55 and p75 subunit of interleukin-2 receptor on the peripheral blood mononuclear cells in different phases of Hodgkins disease. British Journal of Haematology 87(Suppl. 1): 217, 1994

Effects of selenium on peripheral blood mononuclear cell membrane fluidity, interleukin-2 production and interleukin-2 receptor expression in patients with chronic hepatitis. World Journal of Gastroenterology 10(23): 3531-3533, 2004

Fasting induces changes in peripheral blood mononuclear cell gene expression profiles related to increases in fatty acid beta-oxidation: functional role of peroxisome proliferator activated receptor alpha in human peripheral blood mononuclear cells. American Journal of Clinical Nutrition 86(5): 1515-1523, 2007

Quantitative expression of Toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides. Journal of Immunology 168(9): 4531-4537, 2002

Differential expression of the interleukin 2 receptor (p75) chain on human peripheral blood natural killer subsets. International Immunology 2(6): 481-486, 1990

Fibrin matrices stimulate procoagulant activity, promote the expression of interleukin-1-beta, and suppress interleukin-1 receptor antagonist in human peripheral blood mononuclear cells. Clinical Research 41(2): 282A, 1993