Gametic selection in a doubled haploid population derived from anther culture of indica/japonica cross of rice

Chen, Y.; Lu, C.; He, P.; Shen, L.; Xu, J.; Xu, Y.; Zhu, L.

Acta Genetica Sinica 24(4): 322-329


Accession: 008716922

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Gametic selection was assessed with a doubled haploid population including 135 lines derived from anther culture of a typical indica and japonica cross, ZYQ 8/JX 17, by investigating the segregation and recombination of morphological characters, isozymes and RFLP markers. Four major quantitative traits and six morphological indices involving characteristics of indica or japonica were surveyed for segregation distortion. The results indicated that the data for all the traits were successive and represented to be normal distribution. The analysis of 8 isozymes from 52 DH lines indicated that except Cat-1 and Est-2, the segregation of 6 isozymes coincided with expected 1:1 ratio. The segregation deviations of Cat-1 and Est-2 were in favor of japonica and indica respectively, while the ratio of total indica and japonica phenotypes was similar each other. One hundred and thirty-two DH lines were assayed using 167 RFLP markers. It was discovered that 36% markers showed clear segregation distortion, while the ratio of distortion in favor of indica was similar to that of japonica. The genome ratio of ZYQ 8 was equal to that of JX 17 in the DH population and various genome composions of represented normal distribution. Thus, although some segregation distortions were observed in the DH population, the segregation and recombination of genes of indica and japonica were random basically and the overall frequency of alleles did not deviate from the ratio of 1:1.