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Generation and characterization of anti-idiotypic antibodies recognizing the interferon-alpha receptor: implications for ligand-receptor interactions

Generation and characterization of anti-idiotypic antibodies recognizing the interferon-alpha receptor: implications for ligand-receptor interactions

Journal of Interferon Research 13(4): 295-301

Monoclonal antibodies LI-1 and LI-8 against interferon-alpha-A (IFN-alpha-A) block IFN-alpha-A activity and binding to its receptor, but they recognize distinct epitopes. Surprisingly, anti-idiotypic antibodies to both LI-1 and LI-8 have properties consistent with recognition of the receptor: anti-LI-1 and anti-LI-8 antibodies inhibit the binding of IFN-alpha-A to its receptor. However, anti-LI-1 is an antagonist of IFN-alpha-A, while anti-LI-8 is an agonist. Thus, at least some part of the epitopes on IFN-alpha-A recognized by LI-1 and LI-8 are directly involved in receptor binding. Because these epitopes are spatially distinct, the implication is that the receptor binding site on IFN-alpha-A must be extensive, or there are minimally two regions of IFN-alpha-A involved in receptor interactions.

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Accession: 008723146

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PMID: 7693831

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