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Genetic analysis of the tetA(C) gene on plasmid pBR322

Genetic analysis of the tetA(C) gene on plasmid pBR322

Journal of Bacteriology 174(24): 7926-7933

The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance were identified at 30 distinct codons. Mutations that encoded substitutions within putative membrane-spanning alpha-helical regions were scattered throughout the gene.

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Accession: 008725135

Download citation: RISBibTeXText

PMID: 1459940

DOI: 10.1128/jb.174.24.7926-7933.1992

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