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Genetic analysis using genomic representations

Genetic analysis using genomic representations

Proceedings Of The National Academy Of Sciences Of The United States Of America. 95(8): 4487-4492, Il 14

Analysis of the genetic changes in human tumors is often problematical because of the presence of normal stroma and the limited availability of pure tumor DNA. However, large amounts of highly reproducible "representations" of tumor and normal genomes can be made by PCR from nanogram amounts of restriction endonuclease cleaved DNA that has been ligated to oligonucleotide adaptors. We show here that representations are useful for many types of genetic analyses, including measuring relative gene copy number, loss of heterozygosity, and comparative genomic hybridization. Representations may be prepared even from sorted nuclei from fixed and archived tumor biopsies.

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Accession: 008725188

Download citation: RISBibTeXText

PMID: 9539764

DOI: 10.2307/44923

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