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Genomic restriction fragment analysis of fowlpox-virus vaccine strain and field isolates using EcoRI, Sal I and Pst I restriction endonuclease



Genomic restriction fragment analysis of fowlpox-virus vaccine strain and field isolates using EcoRI, Sal I and Pst I restriction endonuclease



Indian Journal of Animal Sciences 65(12): 1275-1280



Fowl-pox-virus IVRI vaccine strain and 2 field isolates were propagated in chicken-embryofibroblast-cell culture and purified by sucrose gradient ultracentrifugation. Transmission electronmicroscopy revealed the purified virus showing characteristic fowl-pox-virus morphology with bilayer structure and surface tubular elements. The viral DNA was extracted and analysed after digestion with EcoRI, Sal I and Pst I restriction endonucleases. EcoRI digestion produced 24 well resolved fragments with some comigrating fragments and analysis on a separate gel revealed 40 restriction fragments. Sal I and Pst I digestion revealed a large unresolved fragments with 4 and 18 well resolved fragments respectively. The 3 viruses showed similar electrophoretic pattern of DNA restriction fragments indicating them to be genetically almost similar.

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Accession: 008732300

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