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Growth and competition between two strains of Bradyrhizobium japonicum in broth and in a peat-based inoculant: Dinitrogen fixation efficiency and competition for nodulation sites



Growth and competition between two strains of Bradyrhizobium japonicum in broth and in a peat-based inoculant: Dinitrogen fixation efficiency and competition for nodulation sites



Revista de Microbiologia 26(3): 211-218



Bradyrhizobium japonicum strains SEMIA 587 and SEMIA 5019 were evaluated for growth and competition in liquid broth cultures and in sterilized peat. Nodulation ability, strain nodule occupancy rates and dinitrogen fixation were investigated in Leonard jar experiments using soybean plants as host. B. japonicum strains SEMIA 587 and 5019 were grown together in a bench-top fermentor or separately in shake-flask cultures, using glycerol as primary carbon source. Maximum growth rate and generation time occurred at 28 degree C and 250 rpm. Maximum productivity was at 28 degree C and 300 rpm. Shake-flask cultures inoculated with equal numbers of both strains contained 69% of strain 587 and 31% of strain 5019 after 120 hours (double-strain broth), due to the lower growth rate and productivity of strain 5019. When double-strain broth was added to sterilized peat only 10.2% of strain 5019 was recovered after 180 days of incubation, but when sterilized peat was inoculated with a 1:2 (v/v) mixture of the strains, 37% of strain 5019 was recovered after the same time period. Total cell numbers increased during 30 days of incubation at 24 degree C-25 degree C and then declined. Mixed inoculi containing both strains generally showed higher nodule occupancy rates for strain 587.

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