+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes



Hibiscus protocatechuic acid protects against oxidative damage induced by tert-butylhydroperoxide in rat primary hepatocytes



Chemico-Biological Interactions. 101(2): 137-148



Hibiscus protocatechuic acid (PCA), a simple phenolic compound isolated from Hibiscus sabdariffa L., was studied for its protective effects against oxidative damage induced by tert-butylhydroperoxide (t-BHP) in a primary culture of rat hepatocytes. It had been reported that exposure of isolated hepatocytes to t-BHP results in leakage of lactate dehydrogenase (LDH) and alanine transaminase (ALT), peroxidation of cellular lipids, and depolarization of mitochondria. The present investigations showed that PCA at concentrations of 0.05 mg/ml and 0.10 mg/ml significantly decreased the leakage of LDH (P lt 0.01) and ALT (P lt 0.05 and P lt 0.01) and the formation of malondialdehyde (MDA; P lt 0.05 and P lt 0.01) induced by 30-min treatment with t-BHP (1.5 mM) in primary cultured rat hepatocytes. PCA also attenuated t-BHP (0.10 mM) induced mitochondrial depolarization as determined by a retention test of rhodamine 123 and DNA repair synthesis as evidenced by unscheduled DNA synthesis (UDS). In addition, PCA exhibited an effective ability to quench 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH). In conclusion, PCA demonstrated protective effects against cytotoxicity and genotoxicity of hepatocytes induced by t-BHP. One of mechanisms of PCA's protective effect may be associated with its property of scavenging free radicals.

(PDF emailed within 0-6 h: $19.90)

Accession: 008770075

Download citation: RISBibTeXText

PMID: 8760395

DOI: 10.1016/0009-2797(96)03721-0


Related references

Protective effects of capillarisin on tert-butylhydroperoxide-induced oxidative damage in rat primary hepatocytes. Archives of Toxicology 73(4-5): 263-268, June-July, 1999

Macelignan protects HepG2 cells against tert-butylhydroperoxide-induced oxidative damage. Biofactors 29(1): 1-10, 2007

Youngia denticulata protects against oxidative damage induced by tert-butylhydroperoxide in HepG2 cells. Journal of Medicinal Food 14(10): 1198-1207, 2012

The effects of Hibiscus protocatechuic acid supplementation on exhaustive exercise induced oxidative stress and muscle damage in rats. Medicine & Science in Sports & Exercise 32(5 Suppl ): S324, 2000

Effect of hypoxia on tert-butylhydroperoxide-induced oxidative injury in hepatocytes. Molecular Pharmacology 34(3): 413-420, 1988

Role of mitochondrial free calcium in oxidative injury induced by tert-butylhydroperoxide in rat hepatocytes. Hepatology 22(4 PART 2): 377A, 1995

Formation of iron-nitrosyl complexes protects K/VP5 cells against tert-butylhydroperoxide-induced oxidative stress. Biochemical Society Transactions 24(4): 536S, 1996

Mechanism of protection by epicatechin against tert-butylhydroperoxide induced oxidative cell injury in isolated rat hepatocytes and calf thymus DNA. Process Biochemistry 37(6): 659-664, January 10, 2002

Alpinia protocatechuic acid protects against oxidative damage in vitro and reduces oxidative stress in vivo. Neuroscience Letters 403(3): 206-210, 2006

An aqueous extract of Rubus chingii fruits protects primary rat hepatocytes against tert-butyl hydroperoxide induced oxidative stress. Life Sciences 72(3): 329-338, December 6, 2002

N-acetylcysteine protects against fluoride-induced oxidative damage in primary rat hepatocytes. Toxicology in Vitro 27(8): 2279-2282, 2014

Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes. Toxicology in Vitro 24(2): 516-522, 2010

Hibiscus protocatechuic acid inhibits lipopolysaccharide-induced rat hepatic damage. Archives of Toxicology 77(1): 42-47, 2002

Protective effect of S-adenosylmethionine on cellular and mitochondrial membranes of rat hepatocytes against tert-butylhydroperoxide-induced injury in primary culture. Chemico-Biological Interactions 156(1): 13-23, 2005

Hibiscus protocatechuic acid supplementation reduces oxidative stress induced by exhaustive exercise in rat muscle. Journal of Exercise Science and Fitness 4(1): 59-64, 2006