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High fertilization rate obtained after intracytoplasmic sperm injection with 100 percent nonmotile spermatozoa selected by using a simple modified hypo-osmotic swelling test



High fertilization rate obtained after intracytoplasmic sperm injection with 100 percent nonmotile spermatozoa selected by using a simple modified hypo-osmotic swelling test



Fertility & Sterility 68(2): 373-375



Objective: To report a high fertilization rate after intracytoplasmic sperm injection (ICSI) in patients with 100% nonmotile spermatozoa selected by a simple modified hypo-osmotic swelling test. Design: Clinical study. Setting: Hospital-based IVF center. Patient(s): Three couples with infertility due to asthenospermia. Intervention(s): The hypo-osmotic swelling test with 150-mOsm NaCl solution was used to select viable spermatozoa before ICSI. Three patients provided semen samples and one of these three also had a testicular biopsy. Main Outcome Measure(s): Selection of viable spermatozoa using the hypo-osmotic swelling test with 150-mOsm NaCl solution for ICSI. Result(s): No motile spermatozoa were found in three ejaculated semen samples and one testicular biopsy. Fifty-seven metaphase-II oocytes were injected with hypo-osmotic swelling test-positive ejaculated or testicular spermatozoa. Fifty-five (96.5%) of these oocytes were intact after injection. Forty-two (76.4%) of 55 oocytes showed two pronuclei, and 40 of the 42 fertilized oocytes cleaved. One patient had all embryos cryopreserved because of the risk of hyperstimulation; two other patients had embryos transferred. One ongoing pregnancy resulted. Conclusion: This hypo-osmotic swelling test with 150-mOsm NaCl solution is a simple and efficient method for selection of viable spermatozoa. A high fertilization rate can be obtained using ICSI with viable spermatozoa selected by using this hypo-osmotic swelling test.

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