+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

High nm23-H1/NDPK-A expression in Ewing tumors: Paradoxical immunohistochemical reactivity and lack of prognostic significance



High nm23-H1/NDPK-A expression in Ewing tumors: Paradoxical immunohistochemical reactivity and lack of prognostic significance



International Journal of Cancer 64(2): 104-111



Expression of nm23-H1/NDPK-A has been reported to correlate inversely with metastasizing potential of rodent experimental cells and some human tumors. In the search for reliable molecular prognostic indicators for Ewing tumors (ET), a group of aggressive presumably neuroectodermal malignancies in children and adolescents, we studied nm23-H1/NDPK-A expression. Northern-blot and RT-PCR analyses were employed to semi-quantificatively measure nm23-H1 mRNA levels in ET cell lines and tissue extracts. A panel of monoclonal antibodies (MAbs) were used to evaluate protein abundance by Western blotting and immunohistochemistry. The nm23-H1/NDPK-A gene was also investigated on the DNA level to define possible genomic alterations. Our results revealed neither nm23-H1 allelic loss nor gene amplification and failed to show any significant variation in nm23-H1 mRNA or NDPK-A protein levels of primary or metastatic ET. NDPK-A protein levels were high and comparable to those of MCF-7 breast-cancer cells and to aggressive stage-IV neuroblastoma cell lines. nm23-H21 NDPK-B expression in ET was slightly more variable but generally lower than in MCF-7 cells. In the immunohistochemical analysis, however, discrepancies in the reactivity patterns with different antibodies were observed. Differential sensitivity to various fixation methods and heat treatment pointed to a structurally polymorphic NDPK-A protein. nm23-H1 expression studies using immunohistochemistry for prognostic counselling should thus be interpreted with caution.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008772066

Download citation: RISBibTeXText

PMID: 7542225

DOI: 10.1002/ijc.2910640206


Related references

Prognostic significance of Nm23/NDPK expression in breast carcinoma, assessed on 10-year follow-up by automated and quantitative immunocytochemical assays. Journal of Pathology 184(4): 401-407, 1998

Immunohistochemical analysis of nm23 gene product/NDPK expression in human lung carcinomas. Zhonghua Jiehe He Huxi Zazhi 18(4): 209-211, 254, 1995

Immunohistochemical analysis of nm23 gene product/NDP kinase expression in pulmonary adenocarcinoma: lack of prognostic value. British Journal of Cancer 66(3): 533-536, 1992

The expression and significance of NDPK/nm23 in gestational trophoblastic tumor. Hubei Yikedaxue Xuebao 20(3): 189-191, July, 1999

Lack of prognostic significance of nm23 expression in human primary breast cancer. Oncology Reports 9(6): 1205-1208, 2002

Prognostic significance of nm23-protein expression in malignant melanoma An immunohistochemical study. Pigment Cell Research 0(SUPPL 5): 47, 1996

nm23 gene product/NDPK expression and its clinical significance in human colorectal carcinoma. Zhonghua Bing Li Xue Za Zhi 24(6): 356-358, 1995

Evaluation of the prognostic significance of nm23/NDP kinase protein expression in cervical carcinoma: an immunohistochemical study. Gynecologic Oncology 61(3): 378-383, 1996

Expression of nm23/NDPK, integrin, type IV collagenase and uPA in transplanted tumors with various metastatic potential in nude mice and surgical specimens. Zhonghua Yi Xue Za Zhi 77(8): 597-600, 1997

Prognostic significance of HLA class I expression in Ewing's sarcoma family of tumors. Journal of Surgical Oncology 103(5): 380-385, 2011

The prognostic relation of nm23-H1/NDPK-A with leukemia and lymphoma. Sheng Li Ke Xue Jin Zhan 34(1): 74-77, 2003

Lack of independent prognostic significance of p21 and p27 expression in advanced ovarian cancer: an immunohistochemical study. Clinical Cancer Research 5(10): 2848-2853, 1999

Lack of prognostic significance of adiponectin immunohistochemical expression in patients with triple-negative breast cancer. Contemporary Oncology 18(1): 34-38, 2014

Evaluation of the prognostic significance of nm23/NDP kinase and cathepsin D in anal carcinomas. An immunohistochemical study. Virchows Archiv 428(2): 85-89, 1996