High-performance liquid chromatographic analysis for the determination of miconazole in human plasma using solid-phase extraction

Kobylińska, M.; Kobylińska, K.; Sobik, B.

Journal of Chromatography. B, Biomedical Applications 685(1): 191-195


ISSN/ISBN: 1572-6495
PMID: 8930770
DOI: 10.1016/0378-4347(96)00125-9
Accession: 008774539

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A high-performance liquid chromatographic method for the determination of miconazole in human plasma is described. A solid-phase extraction was performed on an octadecyl (C-18) cartridge. Miconazole was eluted with methanol, separated on a reversed-phase column and was measured by ultraviolet detection at 230 nm. The absolute extraction recovery from plasma samples was 85%. The limit of detection was established as 5 ng/ml. The coefficient of variation of the determination of plasma levels by this method over the standard curve concentration range was less than 10%, except with the concentration of 10 ng/ml. The plasma levels of miconazole in twelve healthy volunteers given a 250-mg oral dose of two tablet forms were determined by this method.