High-performance liquid chromatographic determination of penicillins by means of automated solid-phase extraction and photochemical degradation with electrochemical detection

Lihl, S.; Rehorek, A.; Petz, M.

Journal of Chromatography A 729(1-2): 229-235


ISSN/ISBN: 0021-9673
PMID: 9004944
DOI: 10.1016/0021-9673(95)00898-5
Accession: 008774787

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An automated precolumn exchange system for on-line solid-phase extraction (OPS-2) coupled to liquid chromatography with photochemical degradation and electrochemical detection was used for the determination of residual amounts of penicillin G, penicillin V, oxacillin, cloxacillin and dicloxacillin. A 5-10-fold increase in sensitivity was obtained when compared with direct UV detection of penicillins at 225 nm. The system is recommended for samples that have received an immunoaffinity clean-up. Analysis of bovine muscle tissue indicated, however, that this procedure could also be suitable for the determination of penicillin G at its maximum residue limit of 50 mu-g/kg even with conventional solvent partitioning for the first clean-up steps.