Highly sensitive detection of gene expression of an intronless gene: amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA)
Heim, A.; Grumbach, I.M.; Zeuke, S.; Top, B.
Nucleic Acids Research 26(9): 2250-2251
1998
ISSN/ISBN: 0305-1048
PMID: 9547289
DOI: 10.1093/nar/26.9.2250
Accession: 008776331
NASBA is an isothermal nucleic acid amplification reaction that amplifies mRNA in a dsDNA background. Although similar to the sensitive reverse transcription/ polymerase chain reaction (RT-PCR) in mRNA detection, NASBA is not prone to give false positive results caused by genomic dsDNA. Therefore, NASBA is unique for sensitive detection of transcription of intronless genes, which preclude strategies such as intron spanning primer pairs to control false positive results in RT-PCR.