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Histamine-stimulated phospholipase C signalling in the adrenal chromaffin cell: effects on inositol phospholipid metabolism and tyrosine hydroxylase phosphorylation



Histamine-stimulated phospholipase C signalling in the adrenal chromaffin cell: effects on inositol phospholipid metabolism and tyrosine hydroxylase phosphorylation



Clinical and Experimental Pharmacology & Physiology 24(8): 624-631



1. The present report gives a detailed account of histaminestimulated phospholipase C (PLC) activity in bovine adrenal chromaffin cells. 2. Histamine activation of H-1 receptors stimulates PLC with a biphasic sensitivity to extracellular Ca-2+. The initial response (the first 15 s stimulation) was not reduced by the removal of extracellular Ca-2+, whereas the maintenance of PLC activity beyond this time required Ca-2+ influx. 3. Phospholipase C activity in response to a 10 min incubation with histamine was inhibited by La-2+ (3 mmol/L) or SKF 96365 (10 mu-mol/L). Nifedipine (10 mu-mol/L), but not omega-agatoxin IVA (100 nmol/L or omega-conotoxin GVIA (300 nmol/L), produced a partial inhibition of PLC activity. The response was also partially inhibited by a reduction in the extracellular Cl- concentration (40 mmol/L) or by the inclusion of the Cl- channel blocker N-phenylanthranilic acid (300 mu-mol/L). 4. Kinetic analysis of the rate of turnover of the various inositol phosphate isomers in response to histamine suggested that the inositol monophosphates were being produced from a source in addition to inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) metabolism. This conclusion was supported by the differential action of pertussis toxin and neomycin on Ins(1,4,5)P-3 formation compared with inositol monophosphate formation. 5. We have attempted to identify a defined role for the intracellular Ca-2+ mobilized in these cells in response to histamine. After short incubations (up to 3 min), histamine was able to regulate the site-specific phosphorylation of tyrosine hydroxylase, the rate-limiting enzyme in catecholamine synthesis. This observation has important implications for a possible role for the PLC signalling pathway in controlling the rate of catecholamine biosynthesis.

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Accession: 008777302

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PMID: 9269539


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