+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

How common are common fragile sites: Variation of aphidicolin-induced chromosomal fragile sites in a population of the deer mouse (Peromyscus maniculatus)

How common are common fragile sites: Variation of aphidicolin-induced chromosomal fragile sites in a population of the deer mouse (Peromyscus maniculatus)

Human Genetics 100(2): 182-188

Aphidicolin (APC)-induced chromosomal gaps and breaks were analyzed for ten deer mice (Peromyscus maniculatus) from a natural population. The FSM statistical methodology was used to identify fragile sites as chromosomal loci exhibiting significantly non-random numbers of gaps/breaks in each individual and enabled an assessment of variation in fragile sites among the individuals. The individual deer mice exhibited as few as 7 to as many as 19 of the populational total of 34 sites. Two sites were fragile in all individuals and 13 sites were fragile in single individuals only. Defined by populational frequencies of greater than 50%, high-frequency fragile sites constituted 26% of the populational total. Approximately 35% of the total fragile sites were fragile in 20-40% of the population (low-frequency fragile sites) and about 38% were fragile in single individuals only. Analysis of the data pooled over all individuals identified significantly non-random breakage at 80 sites, 47 of which were not identified as fragile in any single individual. It appears, therefore, that fragile site identifications from pooled data have fostered an inflated estimate of the numbers and frequencies of common fragile sites. Comparison of the fragile site and spontaneous breakage (control) data suggest that APC-induced fragile sites represent regions of chromosomes that experience elevated levels of somatic mutation. Additionally, the occurrence of APC-induced fragile sites at or near the interstitial breakpoints of two pericentric-inversion polymorphisms in this population supports the hypothesis that fragile sites experience an increased rate of meiotic chromosomal mutation and are predisposed to undergo phylogenetic rearrangement.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 008784193

Download citation: RISBibTeXText

PMID: 9254846

DOI: 10.1007/s004390050487

Related references

How common are common fragile sites in humans: interindividual variation in the distribution of aphidicolin-induced fragile sites. Cytogenetic and Genome Research 101(1): 8-16, 2003

Fibroblast-specific common fragile sites induced by aphidicolin. Human Genetics 83(1): 45-48, 1989

Cancer chromosome breakpoints and common fragile sites induced by aphidicolin. Cancer Genetics and Cytogenetics 13(2): 185-188, 1984

Population cytogenetics of folate-sensitive fragile sites. I. Common fragile sites. Human Genetics 80(4): 344-348, 1988

Common fragile sites induced by folate deprivation, BrdU and aphidicolin: their frequency and distribution in Japanese individuals. Jinrui Idengaku Zasshi. Japanese Journal of Human Genetics 33(3): 355-364, 1988

Increased expression of aphidicolin-induced common fragile sites in Tourette syndrome: the key to understand the genetics of comorbid phenotypes?. American Journal of Medical Genetics 67(1): 25-30, 1996

Reduced expression of aphidicolin-induced common fragile sites in peripheral lymphocyte chromosomes of patients with B-cell chronic lymphocytic leukemia. Cancer Genetics and Cytogenetics 39(1): 99-102, 1989

Synergistic effect of aphidicolin and ethanol on the induction of common fragile sites. Human Genetics 75(1): 75-78, 1987

Identification of the human/mouse syntenic common fragile site FRA7K/Fra12C1--relation of FRA7K and other human common fragile sites on chromosome 7 to evolutionary breakpoints. International Journal of Cancer 120(1): 48-54, 2006

Aphidicolin-sensitive specific common fragile sites: a biomarker of exposure to pesticides. Environmental and Molecular Mutagenesis 29(3): 250-255, 1997

Expression of common fragile sites in untreated non-Hodgkin's lymphoma with aphidicolin and folate deficiency. Cancer Letters 86(1): 111-117, 1994

Expression of folate sensitive and aphidicolin induced chromosomal fragile sites in familial neuroblastoma. Journal of Experimental and Clinical Cancer Research 21(3): 383-388, 2002

Population cytogenetics of aphidicolin-induced fragile sites. Human Genetics 89(5): 543-547, 1992

DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes. Human Genetics 67(2): 136-142, 1984

The Expression of Common Fragile Sites in Peripheral Blood Lymphocytes of Breast and Colorectal Cancer Patients with Aphidicolin. The Tohoku Journal of Experimental Medicine 189(2): 107-116, 1999