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Hydrophobicity and water activity relationships of water-miscible aprotic solvents on kyotorphin synthesis catalyzed by alpha-chymotrypsin



Hydrophobicity and water activity relationships of water-miscible aprotic solvents on kyotorphin synthesis catalyzed by alpha-chymotrypsin



Biotechnology Letters 19(10): 1005–1009



alpha-Chymotrypsin was used to synthesize a kyotorphin derivative in water-miscible organic media. Increase in solvent mole fraction deactivated the enzyme exponentially. Maximum synthetic activity was with a water activity in the medium of higher than 0.8 for all assayed solvents. The increase in solvent hydrophobicity (log Pi) (being Aw higher than 0.8) enhanced 2-times both the selectivity of the synthetic reaction, and the fluorescence intensity and emission wavelength of the enzyme, without dependence on the nature of solvent.

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Accession: 008793791

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