+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of signals required for the insertion of heterologous genome segments into the reovirus genome

Identification of signals required for the insertion of heterologous genome segments into the reovirus genome

Proceedings of the National Academy of Sciences of the United States of America 92(26): 12362-6

In cells simultaneously infected with any two of the three reovirus serotypes ST1, ST2, and ST3, up to 15% of the yields are intertypic reassortants that contain all possible combinations of parental genome segments. We have now found that not all genome segments in reassortants are wild type. In reassortants that possess more ST1 than ST3 genome segments, all ST1 genome segments appear to be wild type, but the incoming ST3 genome segments possess mutations that make them more similar to the ST1 genome segments that they replace. In reassortants resulting from crosses of the more distantly related ST3 and ST2 viruses that possess a majority of ST3 genome segments, all incoming ST2 genome segments are wild type, but the ST3 S4 genome segment possesses two mutations, G74 to A and G624 to A, that function as acceptance signals. Recognition of these signals has far-reaching implications for the construction of reoviruses with novel properties and functions.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 008810201

Download citation: RISBibTeXText

PMID: 8618901

DOI: 10.1073/pnas.92.26.12362

Related references

Identification of sequence elements containing signals for replication and encapsidation of the reovirus M1 genome segment. Virology 186(2): 377-388, 1992

Identification of the 5' sequences required for incorporation of an engineered ssRNA into the Reovirus genome. Virology 329(2): 348-360, 2004

Studies on reovirus RNA. II. Characterization of reovirus messenger RNA and of the genome RNA segments from which it is transcribed. Journal of Molecular Biology 29(1): 19-26, 1967

The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes. Virology 169(1): 194-203, 1989

Sequential partially overlapping gene arrangement in the tricistronic S1 genome segments of avian reovirus and Nelson Bay reovirus: implications for translation initiation. Journal of Virology 76(2): 609-618, 2002

Sequential Partially Overlapping Gene Arrangement in the Tricistronic S1 Genome Segments of Avian Reovirus and Nelson Bay Reovirus: Implications for Translation Initiation. Journal of Virology 76(2): 609-618, 2002

What reassorts when reovirus genome segments reassort?. Journal of Biological Chemistry 270(9): 4181-4184, 1995

Reovirus genome RNA segments: resistance to S-1 nuclease. Virology 64(1): 96, 1975

An identical 3'-terminal sequence in the ten reovirus genome RNA segments. Biochemical and Biophysical Research Communications 45(6): 1518-1525, 1971

Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. Journal of Virology 2(10): 986-991, 1968

Identification of cis-acting signals in the giardiavirus (GLV) genome required for expression of firefly luciferase in Giardia lamblia. Rna 2(8): 824-834, 1996

Comparisons of the M1 genome segments and encoded mu2 proteins of different reovirus isolates. Virology Journal 1: 6, 2004

Sequence analysis of 12 genome segments of mud crab reovirus (MCRV). Virology 422(2): 185-194, 2012

Characterization of M-class genome segments of muscovy duck reovirus S14. Virus Research 125(1): 42-53, 2007

Genome mining of the Streptomyces avermitilis genome and development of genome-minimized hosts for heterologous expression of biosynthetic gene clusters. Journal of Industrial Microbiology and Biotechnology 41(2): 233-250, 2014