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Identification of signals required for the insertion of heterologous genome segments into the reovirus genome



Identification of signals required for the insertion of heterologous genome segments into the reovirus genome



Proceedings of the National Academy of Sciences of the United States of America 92(26): 12362-6



In cells simultaneously infected with any two of the three reovirus serotypes ST1, ST2, and ST3, up to 15% of the yields are intertypic reassortants that contain all possible combinations of parental genome segments. We have now found that not all genome segments in reassortants are wild type. In reassortants that possess more ST1 than ST3 genome segments, all ST1 genome segments appear to be wild type, but the incoming ST3 genome segments possess mutations that make them more similar to the ST1 genome segments that they replace. In reassortants resulting from crosses of the more distantly related ST3 and ST2 viruses that possess a majority of ST3 genome segments, all incoming ST2 genome segments are wild type, but the ST3 S4 genome segment possesses two mutations, G74 to A and G624 to A, that function as acceptance signals. Recognition of these signals has far-reaching implications for the construction of reoviruses with novel properties and functions.

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Accession: 008810201

Download citation: RISBibTeXText

PMID: 8618901

DOI: 10.1073/pnas.92.26.12362


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