+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Immunohistochemical analysis of p53 expression in malignant lymphomas



Immunohistochemical analysis of p53 expression in malignant lymphomas



American Journal of Pathology 141(6): 1343-1348



p53 is a tumor suppressor gene that commonly undergoes mutations in human tumors, including lymphomas. Because p53 mutations are not restricted to a single locus, immunohistochemistry is useful to detect p53 expression and correlate this finding with lymphoma phenotype. Cryostat sections from 125 cases of lymphoma were analyzed for p53 expression using three different monoclonal antibodies (pAb 421, 1801, 240) which react with human cellular p53 and a common conformational epitope on mutant p53. A control antibody (pAb 246) reacts only with wild type p53 of murine origin and was negative in all cases. Tissue from 29 cases of lymphoid hyperplasia, including six from human immunodeficiency virus-positive (HIV+) patients, were negative for p53. p53 was predominantly localized in nuclei of high-grade lymphomas, including 14 of 46 cases of B cell immunoblastic lymphomas and two of five T cell immunoblastic lymphomas. p53 expression was relatively common in lymphomas from HIV+ patients, and unusual in intermediate and low-grade lymphomas of follicular center cell type. Low-grade lymphoma of small lymphocytic type disclosed p53+ large cells (paraimmunoblasts) that may play a role in tumor progression in this lymphoma subtype. p53 was also strongly expressed in the nuclei of Reed Sternberg cells from 19 of 37 cases of Hodgkin's disease, including six cases of mixed cellularity, and 13 cases of nodular sclerosing type. Immunohistochemical staining is a rapid method to identify p53 expression in lymphomas.

(PDF emailed within 1 workday: $29.90)

Accession: 008819959

Download citation: RISBibTeXText

PMID: 1466398


Related references

Immunohistochemical analysis of adhesion molecules and matrix metalloproteinases in malignant CNS lymphomas: a study comparing primary CNS malignant and CNS intravascular lymphomas. Brain Tumor Pathology 25(2): 73-78, 2008

Immunohistochemical and SSCP analysis of p53 in malignant lymphomas. Journal of Korean Medical Science 13(4): 361-368, 1998

Immunohistochemical studies of protein kinase D (PKD) 2 expression in malignant human lymphomas. Experimental Oncology 28(3): 225-230, 2006

Immunohistochemical analysis of malignant lymphomas with monoclonal antibodies. Journal of Leukocyte Biology 36(2): 249, 1984

Expression of interleukin-1 receptor antagonist protein in malignant lymphomas. An immunohistochemical study. Archives of Pathology & Laboratory Medicine 119(3): 247-251, 1995

Nasopharyngeal carcinomas and malignant lymphomas: an immunohistochemical analysis of 74 cases. Laryngoscope 100(7): 742-748, 1990

Primary malignant lymphomas of the spleen. A morphologic and immunohistochemical analysis of 17 cases. Cancer 66(12): 2612-2619, 1990

A rational approach to immunohistochemical analysis of malignant lymphomas on paraffin wax sections. Journal of Clinical Pathology 50(1): 2-4, 1997

Development of stroma in malignant lymphomas of the brain compared with epidural lymphomas. An immunohistochemical study. Acta Neuropathologica 71(1-2): 125-129, 1986

Immunohistochemical analysis of the ICE/Ced-3 family protease, CPP32 in reactive lymph nodes and malignant lymphomas. Laboratory Investigation 76(1): 125A, 1997

Immunohistochemical molecular and cytogenetic analysis of a consecutive series of 20 peripheral t cell lymphomas and lymphomas of uncertain lineage including 12 ki i positive lymphomas. Genes Chromosomes & Cancer 2(1): 27-35, 1990

Paraffin-immunohistochemical analysis of 226 non-Hodgkin's malignant lymphomas in the endemic area of human T-cell leukemia virus type 1. Acta Pathologica Japonica 41(5): 350-362, 1991

Lymphomas and pseudo lymphomas of the gastrointestinal tract a retrospective analysis including a study of immunologic markers in fifty two malignant lymphomas and five pseudo lymphomas. Archives d'Anatomie et de Cytologie Pathologiques 34(3): 143-145, 1986