+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Immunolocalization of fibronectin during the early response of dog dental pulp to demineralized dentine or calcium hydroxide-containing cement



Immunolocalization of fibronectin during the early response of dog dental pulp to demineralized dentine or calcium hydroxide-containing cement



Archives Of Oral Biology. 40(1): 23-31



The role of fibronectin during the events initiating the post-developmental histogenesis of dentine was investigated by exposing the pulp to implants of autogenous demineralized dentine or calcium hydroxide-containing cement for short periods. Implants exposed for 3 days were processed for immunoelectron-microscopic analysis of fibronectin adsorption on to their surfaces. The localization of fibronectin in the critical area of interaction was examined by immunofluorescence 6, 14 and 21 days after implantation. Heavy adsorption of fibronectin on to the dentine implants and the crystalline structures that had been deposited on the cement implants was demonstrated. Positive fluorescence was consistently seen around dentine implants. Strongly immunopositive fibroblast-like cells and weakly reactive, differentiating odontoblast-like cells were found in association with the implanted matrix. Uncalcified matrix secreted by the polarized or non-polarized cells was consistently rich in fibronectin. Fibroblast-like cells exhibiting intense immunoreaction only at 14 and 21 days were mainly associated with the crystalline precipitates on the cement surfaces or within the surrounding pulp. The findings indicate that the specific inductive effects of demineralized dentine on pulp cells are initiated by exposure of the pulp to a fibronectin-containing surface; adhesion of pulp cells and synthesis of a fibronectin-rich matrix characterize the development of new dentine. The reparative response to non-specific inductive influences such as calcium hydroxide seems to be mediated by progressive enhancement of fibronectin synthesis in pulp cells.

(PDF emailed within 0-6 h: $19.90)

Accession: 008822394

Download citation: RISBibTeXText

PMID: 7748109

DOI: 10.1016/0003-9969(94)00148-5


Related references

Calcium ion release from calcium hydroxide stimulated fibronectin gene expression in dental pulp cells and the differentiation of dental pulp cells to mineralized tissue forming cells by fibronectin. International Endodontic Journal 41(11): 933-938, 2009

Immunolocalization of fibronectin during reparative dentinogenesis in human teeth after pulp capping with calcium hydroxide. Journal of Dental Research 75(8): 1590-1597, 1996

Immunolocalization of fibronectin during reparative dentinogenesis in rat molor teeth after pulp capping with mineral trioxide aggregate or calcium hydroxide. New York State Dental Journal 77(6): 36-42, 2012

Short-term dentinogenic response of dog dental pulp tissue after its induction by demineralized or native dentine, or predentine. Archives of Oral Biology 37(2): 119-128, 1992

Dental pulp pH under silicate cement and calcium hydroxide dressings. Israel Journal of Medical Sciences 5(3): 352-359, 1969

Pulp response to a novel adhesive calcium hydroxide based cement. European Journal of Prosthodontics and Restorative Dentistry 3(1): 27-32, 1994

The status of dog dental pulp after preparation for metalloceramic crowns using temporary fixative cement containing calcium hydroxide. Stomatologiia 83(5): 10-13, 2004

Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement. Archives of Oral Biology 36(2): 117-128, 1991

A comparative study on dental pulp response to calcium hydroxide, white and grey mineral trioxide aggregate as pulp capping agents. Journal of Conservative Dentistry 14(4): 351-355, 2011

Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement. Dental Traumatology 17(4): 163-166, 2001

Proliferation of rat molar pulp cells after direct pulp capping with dentine adhesive and calcium hydroxide. Clinical Oral Investigations 15(4): 577-587, 2011

Histologic response and tenascin and fibronectin expression after pulp capping in pig primary teeth with mineral trioxide aggregate or calcium hydroxide. Operative Dentistry 36(4): 448-456, 2011

Response of human dental pulp capped with MTA and calcium hydroxide powder. Operative Dentistry 33(5): 488-495, 2008

Effects of hemostatic agents on the histomorphologic response of human dental pulp capped with calcium hydroxide. Quintessence International 38(10): 843-852, 2008

Pulp and dentine usage test on a new dental material-SC-1 high strength glass ionomer cement. Fudan Xuebao (Yixueban) 35(2): 286-289, 2008