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Improved high performance liquid chromatographic method for the determination of tris(hydroxymethyl)aminomethane (THAM) in human plasma, erythrocytes, and whole blood



Improved high performance liquid chromatographic method for the determination of tris(hydroxymethyl)aminomethane (THAM) in human plasma, erythrocytes, and whole blood



Journal of Liquid Chromatography and Related Technologies 21(11): 1701-1715



A sensitive high performance liquid chromatographic (HPLC) assay was developed to quantitate the organic amine proton-acceptor, tris(hydroxymethyl)aminomethane (THAM) in human plasma, erythrocytes, and whole blood. An aliquot of the sample was heat evaporated (200degreeC for 2 h), derivatized, extracted, and then injected onto a symmetry C8 column. The UV absorbance of the effluent was monitored at 237 nm. 2-Amino-2-methyl-1,3-propanediol was found to be an appropriate internal standard. The method has been applied to quantitate the samples from patients undergoing orthotopic liver transplantation. The assay has also been employed to assess the in vitro time of THAM-uptake into the erythrocytes from blood samples incubated at 37degreeC.

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Accession: 008831053

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DOI: 10.1080/10826079808001253


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