EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Improved method of determining the susceptibility of Candida albicans to fluconazole


Improved method of determining the susceptibility of Candida albicans to fluconazole



Journal of Antimicrobial ChemoTherapy 35(1): 155-159



ISSN/ISBN: 0305-7453

PMID: 7768763

DOI: 10.1093/jac/35.1.155

The MICs of fluconazole for four reference strains of Candida albicans were determined in one laboratory by the macrobroth dilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) and by a modified version of the method which incorporated microbroth dilution, RPMI 1640 medium supplemented with glucose and the reading of MIC endpoints by spectrophotometry. The results obtained by the two methods were within one two-fold dilution. In addition, the susceptibilities of 58 clinical isolates of C. albicans to fluconazole were determined in two laboratories by the modified NCCLS method. Close interlaboratory agreement was observed, 98.2% of MICs varying by no more than one two-fold dilution.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008831268

Download citation: RISBibTeXText

Related references

Improved medium for fluconazole susceptibility testing of Candida albicans. Antimicrobial Agents and ChemoTherapy 38(1): 45-48, 1994

Evaluation of a spectrophotometric broth microdilution method for determining fluconazole susceptibility of Candida albicans: influence of RPMI and RPMI-2% glucose on the selection of endpoint criteria. Korean Journal of Laboratory Medicine 22(3): 188-195, 2002

Comparative evaluation of macrodilution and chromogenic agar screening for determining fluconazole susceptibility of Candida albicans. Journal of Clinical Microbiology 34(12): 3237-3239, 1996

Disk diffusion method for fluconazole susceptibility testing of Candida albicans strains. Journal of ChemoTherapy 13(2): 161-166, 2001

Novel fluorescent broth microdilution method for fluconazole susceptibility testing of Candida albicans. Journal of Clinical Microbiology 39(7): 2708-2712, 2001

Quantitation of ergosterol content: novel method for determination of fluconazole susceptibility of Candida albicans. Journal of Clinical Microbiology 37(10): 3332-3337, 1999

The value of the agar dilution method as a screening test for determining the sensitivity of Candida albicans to fluconazole. Mikrobiyoloji Bulteni 36(3-4): 317-322, 2002

Time-Kill Kinetics of Rezafungin (CD101) in Vagina-Simulative Medium for Fluconazole-Susceptible and Fluconazole-Resistant Candida albicans and Non- albicans Candida Species. Infectious Diseases in Obstetrics and Gynecology 2018: 7040498, 2018

Fluconazole therapy of oropharyngeal candidiasis in a patient with multiple endocrine failure does not correlate with Candida albicans susceptibility to fluconazole in vitro. Journal of Medical and Veterinary Mycology: Bi-Monthly Publication of the International Society for Human and Animal Mycology 34(3): 205-208, 1996

Fluconazole susceptibility testing of Candida albicans: microtiter method that is independent of inoculum size, temperature, and time of reading. Antimicrobial Agents and ChemoTherapy 35(8): 1641-1646, 1991

Reduced fluconazole susceptibility of Candida albicans isolates in women with recurrent vulvovaginal candidiasis: effects of long-term fluconazole therapy. Diagnostic Microbiology and Infectious Disease 64(3): 354-356, 2009

Comparison of the sensititre yeastone colorimetric method with the NCCLS broth microdilution modified reference method for amphotericin B, fluconazole, itraconazole and voriconazole susceptibility testing against Candida non-albicans species. Journal de Mycologie Medicale 13(4): 168-174, 2003

Susceptibility of voriconazole on archived fluconazole-resistant Candida albicans and Candida krusei since 1984. Abstracts of the General Meeting of the American Society for Microbiology 99: 320, 1999

Comparison of a spectrophotometric microdilution method with RPMI-2% glucose with the National Committee for Clinical Laboratory Standards reference macrodilution method M27-P for in vitro susceptibility testing of amphotericin B, flucytosine, and fluconazole against Candida albicans. Antimicrobial Agents and ChemoTherapy 40(9): 1998-2003, 1996

Comparison of a spectrophotometric microdilution method with Rpmi-2% glucose with the National Committee for Clinical Laboratory Standards reference macrodilution method M27-P for in vitro susceptibility testing of amphotericin B, flucytosine, and fluconazole against Candida albicans. Antimicrobial Agents and Chemotherapy 40(9): 1998-2003, 1996